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Showing: 21 - 40 of 77 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Backbones Flag Epitope tag pcDNA3 Flag HA - N-terminal Flag-HA for mammalian expression... TEV-His6-Flag for bacterial expression pET Flag TEV - N-terminal Flag-TEV for bacterial... FLAG and SV40 nuclear localization signal Myr Membrane Localization pWZL-Neo-Myr-Flag-DEST - ...Empty Backbones Mammalian CMV, SV40, EF1a, CAG pSG5L Flag HA - Transient expression pBABE-puro...fusing your protein to an epitope tag, such as Flag or HA, will allow you to easily identify your...bacterial expression pCS2FLAG - N or C-terminal 2xFLAG tag in pCS for a variety of systems...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and...lacunosum moleculare were better resolved with smFP_FLAG than GFP.   3. Enhanced ability to distinguish...

  9. Genetic Code Expansion

    Type
    Collection
    ...TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri...154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser 154768 pAS_4xG1 PylT FLAG-G1 PylRS...140018 pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG tyrosyl-tRNA synthetase E. coli Mammalian TAG Simon Elsaesser 140019...Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian... Simon Elsaesser 154773 pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian...TAG Simon Elsaesser 154774 pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser 140012 pAS_4xMx PylT_FLAG-Mx PylRS...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...

  11. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...Gene/Insert Promoter PI Publication 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation...

  12. Huang Lab CRISPR Plasmids

    Type
    Collection
    ...via the links below: ID Plasmid 44758 pST1374-NLS-flag-linker-Cas9 : A human codon-optimized Cas9 expression...

  17. TALEN Expression Vectors

    Type
    Collection
    ... TALENs expressed from these vectors also have a FLAG epitope tag and nuclear localization signal at their...

  19. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...the nuclease-null dCas9 (D10A, H840A) with HA and FLAG epitope tags either alone or fused to the transcriptional...
Showing: 21 - 40 of 77 results