...talents and expertise Fortunately, the eLife ambassadors group was already thinking about ways to tackle... postdoc at Berkeley at the time and an eLife ambassador, had previous experience organizing open access...potentially enable reproducibility practices being adopted by a wider group of researchers. Generating material...

...recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave...

...consistently showing rapid development and quick adoption by the community. Our depositing labs continue... and use new tools. Because there’s such rapid adoption, we thought that requests would be biased towards...tools that fill needs in the community. The rapid adoption of new tools, illustrated in Figure 2, and the...

...marker of neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison... variable without the behavioral test. Another readout may be necessary to prove inhibition of neuronal...Monitoring behavioral output The appropriate behavioral readout for your optogenetics experiment is contained within...

...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

...the cellular location of proteins, and can be a readout for protein interaction studies such as immunoprecipitation...and after treatment.  Westerns are also a useful readout for a number of cellular assays. They can determine...following immunoprecipitation and are a common readout for subcellular fractionations, a procedure that...

...Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein 1 SCRIB Scribbled...

...is a PhD trained scientist at the University of Colorado, Anschutz Medical Campus.  He studies viruses ...currently a postdoctoral fellow at the University of Colorado Anschutz Medical Campus in Aurora, CO where she.... Erin is also the President of Project Bridge Colorado, an advocacy initiative that seeks to bridge the...

...resources, methods, and results that are being adopted in the field. Read on for a brief introduction ...

...NHEJ repaired DSBs): Provides a semi-quantitative readout of the percentage of alleles that have been mutated...

...window) Tovote P, Esposito MS, Botta P, Chaudun F, Fadok JP, Markovic M, Wolff SB, Ramakrishnan C, Fenno ...

...Neurodegenerative and brain disorders are frequently overshadowed by other medical conditions, so these topics... The Addictive Brain, Dr. Sadangi is an eLife ambassador, SfN community leader, and an early research ...

...Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological Material Transfer...by the National Institutes of Health (NIH) and adopted by the Association of University Technology Managers...

...Less than 9% of plastics are ever recycled, so adopting a few meaningful changes in the lab could make...to make research more environmentally friendly. Adopt one or all of the recycling practices below. Reuse...

...science. “It ended up we moved to Colorado for my husband’s postdoc. Colorado is growing in the biotech sphere...

...express a luciferase reporter which is used as a readout for infection of ACE2-expressing target cells. ... MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G,...

... Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers...

...Activate the signaling molecule and perform the assay readout.  Of course, this is much easier said than done...the samples, antibodies, activation, and assay readout used. You’ll note that the secondary antibodies...

...to take. Decide which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden... has to be optimized according to the strategy adopted, and to the specific reaction you are running. ...

...sequential excitations wavelengths as determined by Rosado et al: see Table 2 for more details on the effect...variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott...