...Gene Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including...reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an ...the serotype of AAV preparations.  Using viral genome sequencing to determine serotype AAV packaging is ...LH (2017) AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations...post on AAV titers Browse viral vectors protocols and tips Resources on Addgene.org Find AAVs at Addgene...platform to accommodate adeno-associated viral vector (AAV) samples and created a simplified process, termed...VGS), to confirm the identity and serotype of our AAV preparations. We recently published a description...

... experiments with AAV: 1. Getting your viral vector into your cell of interest: AAV tropism and route ...administration AAV tropism is defined by the viral capsid proteins that determine the AAV serotype. During...characteristics of select AAV serotypes in Table 2 from the paper. Delivery of AAV into the brain can usually...our recently launched AAV Data Hub, an open platform where researchers using AAV from our viral service... Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about our viral...With the help of adeno-associated viral vectors (AAV), neuroscientists can combine classical genetic tools...experiments! Late last year, we hosted our first AAV Education and Development workshop aimed at brainstorming...

.... Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...pGP-AAV-syn-jGCaMP7b-WPRE 104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE ...Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE...some of the AAV we have released in the last few months. You can also browse our entire AAV inventory. ... control AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your ...26976 AAV5 pAAV-hSyn-hChR2(H134R)-mCherry 26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP   Control AAV Control... 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter ...

...viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also ...Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre...pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases...serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New serotype AAV-CamKIIa-jGCaMP8s-WPRE...New viral prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2...pGP-AAV-GFAP-iGABASnFR2-WPRE AAV1, AAV5 Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-GFAP-iGABASnFR2...

...questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>4.5 kB) by AAV? A: One potential... make an efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It ...our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should...samples within a project. Targeting Your AAV Q4: Are there any AAVs that have successful transduction in ...animal models. All of our engineered AAV are presently built on an AAV9 backbone and so will very likely ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...however, that this will require co-delivery of two AAVs at a high MOI, often resulting in a drop in transduction...

...together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this...co-infection of two AAVs. Cas9 orthologs: shorter, but just as potent and specific? The two AAV strategies described...for in vivo studies. Testing AAV-SaCas9 in vivo To test the efficiency of AAV-SaCas9 in vivo, the Zhang ...not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome...proteins with AAV, at just 1,108 and 986 amino acids, respectively, the size of both is within AAV’s packaging...in length, allowing them to be packaged in AAV.   Split AAV approaches  While finding smaller Cas9 orthologs...that the field has overcome with split AAVs. In general split AAVs break a large transgene in two pieces...

...and Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!...with AAV vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors... answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...was: What AAV vector is best for my specific system?  Sharing data and community concerns AAV vectors ...transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(... Fig. 1: Testing of three different AAV serotypes (AAV1, 8, 9) injected into the A2 noradrenergic...Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists...

...useful, DREADD-containing AAV here. But the top requested AAV that became available in 2017 is pmSyn1-EBFP-Cre...specifically in neurons. AAV Delivered Cre - A useful tool for neuroscientists AAVs are widely used by neuroscientists...Hongkui Zeng’s lab for providing this AAV vector! Additional Resources AAV Titers: Where do they come from...service is going strong, the AAV service has shown incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry...This AAV has had over 150 orders since coming online! pmSyn1-EBFP-Cre Available in serotypes AAV1, AAV5...mCherry was the top requested AAV prep for the 2nd year running, and you can learn more about this useful...AAV5, and AAV retrograde (the most popular form), this viral vector is used to deliver DNA encoding Cre...

... serotype pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-WPRE...Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor...vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB...pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth... AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor Fyhn New serotype pAAV-hSyn-JEDI-2P-Kv-WPRE AAV9 Biosensor St-Pierre...-WPRE  AAV9 Biosensor Looger New serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype ...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral vector...

...Using AAVs AAVs Are Great Tools for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging...PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires... to making AAV packaging a little easier! Click here to download the PEI calculator for AAV packaging ...calculator for AAV packaging Select the plasmid molar ratio you would like to use for your AAV packaging ...Making AAV? Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene...this calculator will make it easier to plan your AAV packaging experiments. Let us know what you think... aav...

...Addgene.org AAV Data Hub AAV Guide AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids...β3 AAV8 Laminin receptor (LR)3 AAV receptor2   AAV9 Terminal N-linked galactose3 AAV receptor2... Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors have both distinctive...Viral Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101...Virus (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the...some AAV9 tools are available as in-stock preparations. AAV retrograde (AAVrg, also known as AAV2-retro...receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor2   AAV2 Heparan sulfate ...

...    Figure 1: AAV toolkit for targeted gene delivery to the brain. The AAV (a) capsid, (b) cargo...precious cargo space in AAV vectors. Dual expression systems, which use two or more AAVs to transduce the same... et al, 2019 AAV-PHP.B2 CNS Mice Yes (LY6A) Deverman et al., 2016 AAV-PHP.B3 CNS Mice...2022 AAV-PHP.eC CNS: neurons and astrocytes Mice No (LY6C1) Shay et al., 2023 AAV-PHP.V1...al., 2020 AAV-PHP.N CNS: neurons Mice Yes (LY6A) Kumar et al., 2020 AAV-PHP.B4 CNS ...2023 AAV-F CNS: neurons and astrocytes Mice No (LY6C1)   Hanlon et al., 2019 AAV-BI-28 ...from other labs. AAVs not otherwise attributed were deposited by the Gradinaru lab.  AAV-PHP.B was the first...

...time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer... of ~9 kb into AAV. They found that in defiance of the packaging limit dogma, this AAV successfully mediated...size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split AAV vector approaches...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments reassemble...Addgene.org: Check out the Addgene AAV guide Find ready-to-use AAV preps from Addgene's viral service ...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ...how do you fit a big gene into a tiny vector like AAV? By breaking the transgene into smaller pieces. Reassembly...

...Adeno-Associated Virus (AAV) for cell and gene therapy AAVs for genome editing AAV: A versatile tool for...pigmented epithelium (RPE). AAVs, on the other hand, have broad tropism in the eye. AAV-2, the first identified...occurring and engineered AAV serotypes that further expands the range of AAV properties is available. ...mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors...into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and...technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative disease...and cell) therapies. One may wonder why the first AAV-based gene therapy treatment for a genetic disease...

...Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.... of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the primary...are some of the AAV we have released so far in 2019. You can also browse our entire AAV inventory! Our ...Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent...Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high...iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical activity...114472  AAV5, AAV8  pAAV-hSyn-mCherry 50459  AAVrg  pAAV-hSyn-DIO-mCherry 59462  AAV2  pAAV-CAG-tdTomato...

...Performance of synthetic promoter AAVs in mice All of these synthetic promoter AAVs were first injected into the...  Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell... A toolkit of synthetic AAV promoters The Roska lab’s synthetic promoter AAVs allows for targeted gene...about using AAV for retinal gene therapy Find more about precise neuronal targeting using AAV technologies...posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors...Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure...basic research. To improve cell-type specificity of AAV, work has focused on modifying the outside protein...

... How did AAV-68544 become contaminated? Well, we purposely mixed another AAV vector with AAV-68544 to ...identified, and we will discard any AAV prep we suspect is contaminated. For AAV-68544, the best match for the...). When we blindly analyzed AAV-68544, we were easily able to identify AAV-44632, the contaminant we mixed...because of poor reagent quality. To make sure our AAV vectors are of the highest quality, we undertake ...research. To make sure that adeno-associated viral (AAV) vectors available at Addgene are suitable for in...perform all the standard quality control assays for AAVs. These steps include measuring viral titer and assessing...Why should you care about viral quality control? AAV vectors are produced by scientists - not robots. ...

...expression.     AAV retrograde Unlike traditional dye-based neuronal tracing reagents, AAV particles require....)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it enables scientists to access...look how cool the brain is!" Using retrograde AAV AAVrg can be used to deliver a wide array of genetic...inhibited) by any genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde... can find in AAV Data Hub!) The cortical pathways to the IC were well transduced by AAVrg while other ...difficult. The in vivo workhorse adeno associated virus (AAV) shows relatively low retrograde transport efficiency...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs...

...and re-launched Addgene AAV Data Hub, the place for scientists to share their AAV validation data. The Data...scientists, learn more about the AAV Data Hub, and hear the incredible AAV talks given by Connie Cepko and... of community submissions for Addgene and CLOVER AAV experiments - and we've updated our submission process...Using the Data Hub If you're trying to decide which AAV is right for your experiment, searching the Data ...can even see dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented...Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should!) submit your data...meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  We love negative data, and in fact...

...nervous system (PNS) targeting AAV  Get 1st time AAV user tips from an AAV rookie  Learn about some considerations...Search for AAV vectors to use with your research. Visit Our AAV Guide Page for info on AAV components,...transfer to the rat CNS: AAV PHP. EB vector dose–response and comparison to AAV PHP. B." Gene therapy 25.5...considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals. Resources...components, common uses of AAV, and much more! Find ready-to-use AAV from Addgene or check out protocols for packaging... its parental AAV9 relied on for transducing cells: galactose and the AAV receptor (AAVR). Cell lines ...such as adeno-associated virus (AAV), from reaching their targets. The AAV9 serotype can breach the BBB ...