...providing plasmids to researchers in developing countries. We are proud to introduce the 5 scientists who...molecular biotechnology for improving expression of nutritionally and therapeutically significant proteins. In...Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical...Expression Systems Learn about Global Plasmid Distribution ...

...alteration) Expensive buffers Tris-tricine 2.5–40 kDa Tris-tricine 30 V, 1 hour or 100 V, 1–2 hours...Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours Good for ...Running conditions Pros Cons Tris-glycine 6–400 kDa Tris-glycine 100 V, 1–2 hours Easy and...that Bis and Tris gels run proteins in different patterns, due to their differing chemistries. You therefore...-life; can alter proteins due to high pH Bis-tris 6–400 kDa MES for proteins <40 kDa 180 V, ... protein alteration) Expensive buffers Bis-tris 6–400 kDa MOPS for proteins > 30 kDA 200 V...therefore cannot compare Bis and Tris gels or blots to each other. Once you know your gel type, you’ll need...

... Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii... Mehl 163915 pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial...Huber 177311 pRSF-G1TFAKRS M. archaeon N6-(trifluoroacetyl)-L-lysine (TFA-Lys) Bacterial TAG Thomas Huber...200226 pMega-MaFRSA PylRS M. alvus meta-trifluoromethylphenylalanine Bacterial TAG Alanna Schepartz 201922...

...project We will ship you  2 µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments...this period, we’d love to hear from you if you’ve tried out this service! Let us know what you think. You...excited to begin offering this service to further contribute to our mission to “accelerate research and discovery...

...complex and dynamic field. We’re going to focus on a tried and true method of finding enhancers by isolating...mono-methylated and acetylated histones, but not tri-methylated histones, functions as an enhancer. We... me1, me3, and ac – denoting mono-methylation, tri-methylation, and aceylation, respectively (these ...suppose you have a piece string that’s 10 meters long. You twist and roll the string into a tight ball. Now...This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally...follows: (mono_methylation + acetylation) - tri_methylation In the next section we’ll apply the above formula...Now the two ends of the string, instead of being 10 meters apart, are mere millimeters apart. Similarly...

... an exciting and emotional time!” George Church tries to recruit people who are nice as top criterion ...latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George...

...end up stressed and rushing, no matter how hard I tried to build in extra time before the experiment. What...any experiment. That, more than anything else I tried, made a huge difference in my ability to focus, ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

...and potent liver transduction. While we have not tried it, it might be worth investigating for both Q6 ...cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced CNS tropism to other...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...

...modular code. Use tried-and-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools...tracked. Terminology and language are tricky Names for things will trip everyone up at some point, decide ...messiness of biology. These examples are intentionally trivial, but they're here to help tell this story. In this...plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping. In the...contents might leave you sad and confused when a tricky procedure failed and one of your techs started ...

...biology. While not everything is open-access, I have tried to prioritize open-access resources whenever possible...

...plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates...transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain a chromosomal...presence of trimethoprim, cultures treated with a folA gRNA were more sensitive to trimethoprim when compared... which encodes the target for the antibiotic trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae... the folA gene product not been a target for trimethoprim, there would be no changes in sensitivity. Pooled...

...and lead to you being remembered as a person who tries to find solutions that benefit everyone. Even if...negotiation, I will view the “winner” positively if they tried to understand my position and to find a creative...

...have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator ...the technology in an unethical manner, but I also tried not to be an alarmist.  The overarching guideline...and science communicator, and I strive to be a volunteer and contribute to my community. This past year...all of these roles by reaching out to my school district not just as a dad, but as a scientist. When our...it. PTAs have more flexibility than the school district itself in terms of what they do with their discretionary...like this without them! If you live in a school district that has a lot of parents in STEM careers, chances...chances are they’re wondering how they could contribute to the school, too. Though you can recruit parents...

...month ago. Why haven’t we received our order? I tried searching my PO in the track order portal and nothing... Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the process ...does not own the material in our repository. We distribute it on behalf of depositing institutions. Addgene...

... Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about temperature? Find the ...

...for efficient recombination? A3: The most we've tried to insert so far has been 1kb. We used homology ...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure...

...the grant or your PI may edit everything you’ve tried to draft, but you’ll still be practicing and getting...

...is really important. For my Postdoc position, I tried to read at least 50 papers on the topic before I...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...

...molecules are sensitive to blue light. Many have tried to make green molecules, but then alongside blue...Optogenetics is a toolset that lets you turn the electrical activity of neurons on or off using pulses of...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons...deafness, or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many...popular. Why has it been so important to you to distribute these tools as freely as possible? Boyden: I ...for different ions. Right now, we can modulate electricity. If you could selectively let in calcium or some...brain. Addgene: Do you have any special tips or tricks for someone looking to try these optogenetic tools...

...started learning proper cell culture technique, I tried to find an app that could help me with calculations...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article...comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a double digest...digest tool that is only helpful for NEB restriction enzymes since the instructions use NEB buffers. Nonetheless...name to discover all the information about that restriction enzyme or share it via email with a colleague...colleague. The app is also great if you’re adding a restriction site to a primer, you only need to skim the alphabet...