... human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root..., Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature . 2015 Jan 29;517...

... Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root.... “It was very difficult to screen in a pooled fashion or to go after thousands of targets at once. That...

..., Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP, Halabi C, Sigmund CD, Meyerholz...

...entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing the Cas ...Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright...

..., Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H (2018) In vivo simultaneous...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional...

... Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, ...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...

.../genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step Generation... find that many, if not all, the mice carry a frameshift mutation. For knock in with a donor oligonucleotide... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...

...determine the best concentration for your assay. Washing steps can be done by hand but if available, use...pipette to add 200 µL wash buffer to each well. Washing can be done by hand but if available, use an automated...

...comfortable shoes that cushion your feet. Eye protection such as goggles and/or face shields can be used as needed...Close-toed shoes Gloves Eye protection and face shields, as needed Guidelines Your lab coat should be buttoned...

..., Liu, J., Zhang, H., Xie, L., Ying, W., Li, D., Shi, L., Sun, Y., Li, Y., Li, Y., Sun, Y., & Zuo, E. ...

... 2011. 767: p. 411-8. PubMed PMID: 21822892. 33. Shi, Y., P. Kirwan, and F.J. Livesey, Directed differentiation...thanks to our guest blogger, Kusumika (Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends...This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative... pursue a career in science communication and publishing after completing her postdoctoral training from...441(7097): p. 1061-7. PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells... 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent ...

...stop the exonuclease function of the enzyme and shift its activity back to polymerase. In our case, dGTP...

...Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps container Biohazard...

...the stage arm to move the stage so that the light shines onto your sample. To ensure that your sample is...

...including empty capsids. The 60% step serves as a cushion for genome-containing virions. Phenol red is added...

...protect your skin by wearing safety goggles or a face shield, gloves and a lab coat. Analyzing Your Gel: Using...

...order primers from a company, which synthesizes and ships them as a lyophilized powder. The researcher then...

...very little background staining. Pro-Tip A large shift in the electrophoretic mobility of the bands (sample...

...exchange column. (Optional) Regenerate the column by washing with 25 mL of Protein A/G binding buffer and store...

...scrape and use spreader to scrape plates. Pro-Tip Pushing motion is better than pulling motion Take care ...