...PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...

...system using two cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013... SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression... indicated below) can be cloned into the CRISPR array. The oligos are designed based on the target site..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126...V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;... 10.1016/j.cell.2013.08.021. Epub 2013 Aug 29. Erratum in: Cell. 2013 Oct 10;155(2):479-80. PubMed . Genome...

...programmed cell death" plays a pivotal role in an array of biological processes including development, the...Upon cleavage of the sequence, β10 and β11 can rearrange to their normal antiparallel position and fluoresce...applied to other fluorescent proteins to create an array of multicolor reporters that will enable imaging...dying at the wrong time and/or place can lead to an array of diseases and pathologies such as neurodegenerative...

...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain...

... slides or notes. Listen to their stories and narratives (see below “Tell a story”) and note how they ...the point.   What makes a story? A story is a narrative connecting cause and effect. Stories can and should...obstacles. Take time to consider the order of your narrative. Scientists do this all the time, you just need...Industry Job Talk What’s Your Story? By Herminia Ibarra and Kent Lineback Resources on the Addgene Blog...

...Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini pgRNA-bacteria...interfere S. pyogenes Marraffini pWJ66 46570 Bacteria BsaI yes, activate S. pyogenes Marraffini pWJ68 46571 Bacteria...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868...rapid modular assembly of natural type II-A CRISPR arrays capable of simultaneously repressing multiple target...

... to replicate at a higher frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids...the monomeric version of the plasmid (Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric...monomers and various forms of multimers (Summers & Sharratt, 1984; Summers et al., 1993). In these cases, ...2958.1993.tb01648.x. PMID: 8361350. Summers DK, Sherratt DJ. Multimerization of high copy number plasmids...

...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at...at the Veterans Health Administration. Steph Guerra, PhD, is currently a AAAS Science and Technology Policy...equitable for all. You can follow her on Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse...

...Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein,...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein ...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2-OR1...Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based off of pBESTluc from Promega. Contain...

...estrogen receptor 2 (ER beta) ESRRA Plasmids ESRRA, ERR1, ERRa, ERRalpha, ESRL1, NR3B1 estrogen-related...

...to harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic functions...that can be used to engineer viruses for a wide array of research purposes. Ready-to-use viral preparations...that can be used to engineer viruses for a wide array of research purposes. Which Type of Virus Should...

...Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI...Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter vector...cloning DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH ...

...expressing Cas9 and a gRNA will result in a diverse array of mutations (see: Validate genetic modification... a given genome. Some more recent libraries use arrays of multiple gRNAs to target paralogs or lncRNAs...R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome ...Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G., & Zhang, F. (2013). DNA targeting...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature , 516 (... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...http://en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712...CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819...

...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...

...produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was expressed by some...the restriction enzyme BciVI was present in the aberrant light chain sequence, but was predicted to occur..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG ...

... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering them to these...start from scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, ...

...per each dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By having... histone acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher...Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation...

...finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular... Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc...

...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods...which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods...three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i....