...dLight sensor here! The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar...respectively). Using GRABDA sensors in experiments The Li lab used GRABDA in drosophila, zebrafish, and mouse...

... reliable cloning technique that was developed by Li, et al., in 2011. This method is ligation independent...Biology 38 (2016): 145-154. PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified...

... https://doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the...check out this review article published in Reviews in Medical Virology (Li et a., 2018).   Figure...

...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...of an accessible supply chain for parts that are listed in the bill of material (BOM) of an assembly, the...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

...recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods. 2007 Mar;4(3):251-6....method for sequence- and ligation-independent cloning. Li MZ, Elledge SJ. Methods Mol Biol. 2012;852:51-9. ...

...Constitutive 9 Li 113050 pAAV-hSyn-GRAB_DA1h Syn GRAB_DA1h none Constitutive 9, rg* Li 140553 pAAV-hsyn-GRAB_DA2m...none Constitutive 9 Li 140554 pAAV-hsyn-GRAB_DA2h Syn GRAB_DA2h none Constitutive 9 Li 140555 pAAV-hsyn-...none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m Syn GRAB_rDA1m none Constitutive 9 Li 140557 pAAV-hsyn-GRAB_rDA1h...Constitutive 9 Li 140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208698 pAAV-hSyn-GRAB-gDA3m...Constitutive 9, rg* Li 123309 pAAV-hSyn-GRAB_NE1h Syn GRAB_NE1h none Constitutive 9, rg* Li 123310 pAAV-hSyn-GRAB_NEmut...Constitutive 1, 9 Li 185388 pAAV-hSyn-GRAB_OTmut Syn GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter...pAAV-hSyn-GRAB_ACh3.0 Syn GRAB_ACh3.0 none Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE...

... consider FastCloning, first described by Chaokun Li and colleagues. Here you simply PCR-amplify your ...finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified...number is a bit smaller, let’s say 4 or less. The list goes on... In this post, we’ve reviewed many of ...

...fluorescent dopamine(DA) sensor GRAB_DA1m in neurons Yulong Li 113050-AAV9 pAAV-hSyn-GRAB_DA1h express the genetically-encoded...fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 113050-AAVrg pAAV-hSyn-GRAB_DA1h express the genetically-encoded...fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 115892-AAV8 pAAV-Syn-Archon1-KGC-GFP-ER2 AAV-mediated...norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308-AAVrg pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309-AAV9 pAAV-hSyn-GRAB_NE1h Expresses the genetically-encoded...norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123309-AAVrg pAAV-hSyn-GRAB_NE1h Expresses the genetically-encoded...norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123310-AAV9 pAAV-hSyn-GRAB_NEmut Expresses the genetically-encoded...

... Collections Synthetic Biology Metabolism Synthetic Biology: Metabolism SynBio Resources SynBio Home Browse...Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling...Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort ... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...

...result from excessive nuclease activity in tissues (Li et al., 2018). 4. Selection marker while engineering....e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...that blocks type II-A CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-...

....2014.09.039 Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying...dCas9, also referred to as CRISPRa, was initially published in 2013 (Bikard et al., 2013, Perez-Pinera et ...

...s13039-018-0368-2  Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE,...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of...

... a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the Codon...683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of...

...different leaf infiltration methods Co-cultivation (Li et al., 2009): The co-cultivation of Agrobacterium...Humana Press, 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified...precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily...might help to get you started. Once you have established a well working protocol in your lab, it can become...

...critical that the unknown sample’s concentration lies within the dynamic range of the curve. The dynamic...trendline, which may be good enough if your unknown lies within the linear portion of the graph. It does ...resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control for All ...viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in food; and...BCA assays have linear trendlines while those for ELISAs and other enzymatic assays are sigmoidal. Once ...

...2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin substantially... 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, ...efforts. This holds true for any prime editor experimentalist, and especially shines once large-scale prime...

...Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG,... SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

...past and present, but the real promise of Addgene lies in our future. As we near our 20th anniversary, ...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...

...adjacent motif (PAM). ABE7.10 can edit target 'A' if it lies in a small genomic window: at protospacer position...Nature plants (2018): 1. PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and ...

...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method ...PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted...short list of some other common applications and links to relevant papers and webpages are listed below...such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the... EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay) Southern/Northern... Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation and single-step ...