...Screening Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more... Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries...library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000...SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin...library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000...and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin... in backbone XPR_502 (P65 HSF) targeting 18,885 genes and containing 56,762 unique sgRNAs. This backbone...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable...C. elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...

...regulated gene expression through viral evolution. Zhou X, Vink M, Klaver B, Berkhout B, Das AT. Gene Ther... Tet Plasmids Background To advance the study of gene function, scientists were in search of inducible...inducible promoters capable of controlling eukaryotic gene expression. Several endogenous promoters had been ...is reduced. To use tetracycline as a regulator of gene expression, a tetracycline-controlled transactivator...expression by the activation domain, resulting in reduced gene expression. This idea of a hybrid transactivator... Experimental Tips Choosing a tet system If your gene of interest should be active, and only turned off...tetracycline off or tTA is more appropriate. If your gene of interest should be mostly inactive, and only ...

...knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your gene of ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime... the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter...Selectable Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Synthetic Biology Networks and Gene Regulation Synthetic Biology: Networks and Gene Regulation SynBio Resources...Addgene's collection of synthetic biology plasmids related to Networks and Gene Regulation Plasmid...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...regulatory elements, pre-assembled genetic circuits, and higher level gene networks. Examples Include: promoters...repressors and activators logic gates Networks and Gene Regulation Plasmids Search the table by keyword ...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... a new window) - Tools for gene and protein expression and mapping of gene expression in plants. Figshare...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...plasmids containing promoters that drive general and species-specific gene expression (see the column ‘promoter...vectors: A modular plasmid assembly kit for multigene expression, gene silencing, and silencing rescue in plants...protocol (Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR...a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors...The following collections can be used for studying gene expression in plants: Maize Transcription Factor...

...fold repress on targeted genes. 2. Two-plasmid CRISPRi system for mammalian gene knockdown The first plasmid...sgRNA targeting endogenous CD71 gene 46919 pMLS-SV40-EGFP Target EGFP gene that is stably integrated into... silence transcription on the gene expression level without genetically altering the target loci. Thus...binding platform, to repress expression of arbitrary genes in bacteria or human cells ( Qi et al. ). This CRISPR...designed with a 20-basepair complementary region to any gene of interest. Co-expression of dCas9 and sgRNA can...genome. 1. Two-plasmid CRISPRi system for bacterial gene knockdown The first plasmid ( pdCas9_bacteria ) ...contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector with chloramphenicol resistance...

...knockin. Select gene and genetic element to be manipulated In order to manipulate a given gene using CRISPR...mutant allele of a gene ( point mutant )? Increase or decrease expression of a target gene? Once you have ...sequence for the gene you are trying to target. However, the exact region of the gene you target will ...want to: Generate complete and permanent loss of gene expression or function ( knockout )? Generate a specific... Edit Generate a specific user-defined sequence change in a particular gene, such as generating a point...reporter gene (e.g. GFP) to identify and enrich positive cells, or selection marker to generate stable ...promoter driving expression of your gene of interest. For genetic knockouts, gRNAs commonly target 5′ ...

...knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter...plasmids for transcriptional repression of target genes. CRISPR...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene or sequence...zebrafish-specific gene expression database that provides information about when genes are active in developing...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...-type specific conditional gene inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration... finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model... model. With more than 70% of human disease genes having an ortholog in zebrafish, this species has more...kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish ...

...Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest...Express a gene of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest...biosensors targeting small molecules or specific genes are also available. Custom biosensors can also ...to create a fluorescent fusion protein with your gene of interest and the listed fluorescent protein. ...Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric...monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric...monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused to the C-terminus of monomeric...

...knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...>C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

...a single, short region of a DNA molecule (single gene perhaps) to be copied multiple times by Taq Polymerase...cations can also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the ...

...Transfections allow for transient expression of a gene of interest in cell culture. This protocol describes...Antibodies Blog Posts Molecular Biology Reference General Transfection Protocol Recombinant Antibody Purification...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...intended to support reproducibility in science. General Considerations For antibody-expressing plasmids...

...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily... used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing...which diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in...Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm ( C. elegans, C. briggsae...Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection...

...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs targeted...arrays that can be used to generate ZFNs targeted to a site within that gene. To construct ZFN expression...targeted to a site within that gene. Plasmid...Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene Targets Recent published reports have shown that...tested for activity as ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full ...aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond Syndrome Gene (sbds) OZ545 and OZ546 OPEN OPEN tGTTGCCGTCgtgagGATGAAGAAa...

...Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther. 2013 Dec;13(6)...non-dividing cells. These viruses enable long-term stable gene expression and are permanently integrated into the...-associated viruses (AAVs) are commonly used for gene delivery in-vivo because of their mild immunogencity... as retroviruses, are the most popular virus for gene transfer in clinical trials. These viruses have ... to produce other molecules or to express other genes. Viruses used to direct these alternate functions...for more virus, but instead encode for a specific gene or other research interest. To produce viruses with...those viruses can be used to deliver the "memory" gene (and thus, that protein) to the brain in mice and...

...perspective, sometimes it’s best to delete genes from or introduce new genes and mutations to the bacterial genome... in control of gene expression so be sure to try a few different systems with your gene/protein of interest...Engineering Although overexpressing or knocking down a gene can tell you much about its function and/or alter...you're studying the basic biology of a bacterial gene or redirecting metabolic pathways to produce a therapeutic...Expression of customizable guide RNA (gRNA) for bacterial gene disruption or knockdown. For a more comprehensive...Various Plasmids Tn7 Transposition Nancy Craig A gene of interest cloned into one of these plasmids can...hopefully get a better understanding of what your gene of interest does when expressed in vivo . Plasmid...

...Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells Kotin 65214 ...Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells Kotin 65215 ...Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells Kotin 65216 ...Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells Kotin 92307 ... based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations... to the reliance of the AAV on certain exogenous gene products that mediate AAV replication. This requirement...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g...