... to study these genes! Groucho, a transcriptional co-repressor The first conference room, that is actually...named after the protein Groucho, a transcriptional co-repressor important in development. Groucho family...Ish-Horowicz. "The Groucho/TLE/Grg family of transcriptional co-repressors." Genome biology 9.1 (2008): 205. PubMed...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

... Technique or the Flowtime Technique. Co-work virtually. With co-working, the idea is not necessarily ...

... rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment...from the same incompatibility group should not be co-transformed. +Actual copy number varies. See below...

...determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing the...first round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK...

...basepair complementary region to any gene of interest. Co-expression of dCas9 and sgRNA can efficiently block...designed to target arbitrary sequences in the genome. Co-expression of both plasmids in bacteria could cause...fluorescent gating of transiently transfected cells. Co-expression of both plasmids in HEK293 cells could...

...transgene into the adenoviral plasmid. One can also co-transform standard BJ5183 with supercoiled pAdEasy...series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest....

...all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter...conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein...

...and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs at BWH and providing...is a Junior Faculty at Harvard Medical School/BWH, co-Director of the Mentor Circle Program at BWH, and...health strategy, and global health equity. She is also Co-founder/director of the non-profit foundation IMFAHE...

...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)...

...fluorescent protein tag which let the team detect its co-localization with a membrane-localized mCherry or...protein. With this system, if the OptoNB can bind, it co-localizes with its target at the cell membrane and...

...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo.   Q5: What is the efficiency of co-delivering different AAV particles into the same ...

...Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation...termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes...

...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

...et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a reference...this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous...

...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and...

...postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB workshop and authored a three-part...Global Community Bio Summit at the MIT Media Lab. As a co-founder of a community lab, I realized that not all...

... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ...

...ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify ...ClonTracer to study drug resistance Although Bhang and co-workers started using the tool to look at a variety...

...steady-state complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex...group of proteins, whereas the more loosely bound co-coregulators associate with the core complex in a...