...protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are optimized for excitation... sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...axon-targeted syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype ...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...

...which is limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would...poor scalability.  Scientists at Janelia Research Campus and the University of California, Berkeley engineered...access all the neurons that signaled from the hippocampus to Broca’s area. This could be done by injecting...of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access ...

...technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where he works on developing and optimizing... anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged 14 days post-transfection...completed, Hubbard will image these neurons using GCaMP7, measuring calcium signaling related fluorescence... the screen to understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this...

...need. For example, as shown in this study [3], hippocampal neurons are activated when the mouse is placed...expression, thus showing successful inhibition of hippocampal neurons. Initial monitoring Second, this may ...associated with a visual or auditory cue. The hippocampus has long been known to be responsible for mediating...the function of specific subregions within the hippocampus were only recently studied in detail thanks to...

...culminated in the current President holding and campaigning on many views that don't line up with the scientific...at the University of Colorado, Anschutz Medical Campus.  He studies viruses including HIV and is interested...And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use the same... at the University of Colorado Anschutz Medical Campus in Aurora, CO where she studies the development...

...Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor...Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep ...

...strange to be using a micropipette and gloves at a campsite. And it was exciting – one night while trying ...picnic table before dark, a bison wandered into our campsite. We had to abandon our samples and move farther... Figure 3: When a bison wanders into the campsite, leave your samples behind! Paul and Anastasia...

...Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid...730825. https://doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N...Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. A., Ichiki, T., Mazmanian...Ravindra Kumar, S., Arokiaraj, C. M., Plattner, V. M., Campos, L. J., Mich, J. K., Monet, D., Ngo, V., Ding, ...Scherrer, J., Winchester, J., Blackburn, R. J., Campos, L. J., Man, K. N. M., Sun, J., Chen, X., Lefevre...

...Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus...).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 ...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

...studies have found reduced BDNF levels within the hippocampus and frontal cortex during depression, yet there...injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior...-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats."...

... we’re here to help with our Suggest a Plasmid Campaign. How to suggest a plasmid The scientists at Addgene...reach out to will end up depositing, we hope this campaign will expand the repository in ways that will be...

...optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically...

...Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically...

... at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus of ... at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA.     Richard Gronostajski...

...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ...cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...

...Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Yellow...Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red Calcium Sensors CaMPARI jRCaMP1 jRGECO1 sRGECO Far-Red..., 2, 9 Campbell Calcium Sensor: HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive...Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, ...pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f Syn...soma-jGCaMP8f none Constitutive 1, 9, rg* Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none ...AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Looger 176756 AAV-EF1a-jGCaMP8f-WPRE EF1a...

...biosensor, GCaMP, and click through to the plasmid search results: More than 200 different GCaMP containing...

... disease for which there is currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab...see our interview with Dr. Fujii. References Campbell, Amy E., et al. "NuRD and CAF-1-mediated silencing...

...Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is ...currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would rather...