...as a Grant Professional to mentor and coach you. There are many grant consultants who offer grant training...Associates, Inc., a national grant consulting firm, and Smartegrants, a grant professional development firm... the field of grants. What is a grant professional?  In the past 5-10 years, the Grants Profession has...readiness, identify grant opportunities, secure grant awards, and manage grant projects and funds. You will... for grants, the amount of grant funds available and the increasing regulatory process for grants has ...become a grant professional? Employment opportunities for those with expertise in grant writing, grant management...re seeking a full-time job or want to be an independent grant consultant, building your area of expertise...

...DNA concentration. It’s important to consider the advantages and disadvantages of each method and when...using UV absorbance to quantify DNA Fluorescence dyes Another way to quantify DNA would be to use fluorescent...electrophoresis Quantifying DNA using capillary electrophoresis is similar to quantifying DNA using gel ...fragments are quantified first before larger fragments. Figure 1: The quantification of a RNA ladder...Some last reminders about quantifying DNA In choosing your DNA quantification method there’s many things...UV absorbance is one of the most common ways to quantify DNA. This method involves measuring the absorbance...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally...

...-type. However, variants such as the P.1 (Gamma variant) and the B.1.351 (Beta variant) were much less...B.1.351 variant neutralization escape The lab saw that the B.1.351 variant was very resistant to vaccine...differed depending on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals who received...vaccine could neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429 (Epsilon...on the variant. The lowest levels of cross-neutralization occurs in the P.1 and B.1.351 variants tested...circulating variants. This allowed us to create pseudoviruses that represent lineages and variants that are... distinct from SARS-CoV-2 and its variants. Yet, the B.1.351 variant, which has only 9 different mutations...

...Many Benefits of Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal...Fvasconcellos.     How are Recombinant Antibodies Made? To make recombinant antibodies, you first need to know...definition of recombinant antibodies are essential for good reproducibility. Non-recombinant antibodies are notorious...I predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...the scientific toolbox. What are Recombinant Antibodies? Antibodies are used by scientists around the...Bottom) Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing...recognizing one epitope.   Recombinant antibodies are monoclonal antibodies that are generated in vitro ...

...IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17/SPF45...IgG2a 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...The recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG... by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection The following plasmids...Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222/6R...

...Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids...well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies in the...Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that recognize common.... ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...

...Averof pBUN6A11 50579 Plant BsaI yes, activate S. pyogenes Bar Chen pBUN6I11 50580 Plant BsaI yes, interfere...Bar Chen pBUN411 50581 Plant BsaI yes, cut S. pyogenes Bar Chen pBUN501 50582 Plant BsaI yes, nick S. pyogenes...Chen pHSN6A01 50586 Plant BsaI yes, activate S. pyogenes Hyg Chen pHSN6I01 50587 Plant BsaI yes, interfere...Chen pHSN501 50589 Plant BsaI yes, nick S. pyogenes Hyg Chen pCBC-DT3T4 50592 Plant BsaI none S. pyogenes... Chen pCBC-MT1T2 50593 Plant BsaI none S. pyogenes Chen pCBC-MT2T3 50594 Plant BsaI none S. pyogenes Chen... Chen pCBC-MT3T4 50595 Plant BsaI none S. pyogenes Chen pBUN421 62204 Plant BsaI yes, cut S. pyogenes ...Bar Chen pU3-gRNA 53063 Plant AarI none S. pyogenes Gao pZmU3-gRNA 53061 Plant none S. pyogenes Gao pU6...

...Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns , and Antares : Fluorescent proteins are ...Renilla luciferase (Nano-lanterns) or NanoLuc® (Antares and Enhanced Nano-lanterns) to allow for BRET-based...Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121...systems. Each type of luciferase has advantages and disadvantages depending on the application. Firefly...Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights Empty Backbones Expression...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly ...

...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...PAM sequence often found in plants, expanding the targeting range for plant genome engineering. Read more...more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting...like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...-related antibodies including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-G.... See More Antibodies Find information on Addgene's collection of plasmid-based antibodies, including ...-G [N106/36] Generate anti-kinesin recombinant scFvs . Niwa et al. Cytoskeleton. 2023 Apr 10. Explore ...Explore the recombinant monoclonal antibodies and scFvs for neuroscience research created with high-volume hybridoma... AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...

...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...

...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting neuroscience-related proteins. Antibodies...Neuroscience Antibody Collection Browse or search the table below to find antibodies currently available from...neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Neuroscience Neuroscience...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting epitope tags and common fusion proteins. Antibodies... Other Markers Antibody Collection Browse or search the table below to find antibodies currently available...cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Tags and Other...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

...found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 nucleocapsid...research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid... Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-CoV-2 nucleocapsid...from Ray Owens' lab. Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Abbott...Libraries - Libraries of Spike (S) Ectodomain and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor ...

...Dynamics of Cytosolic Redox Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer... potential Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements...that many of the articles linked below include variants with different binding affinities or negative-...turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. Nat Commun... Calcium Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized...genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain... small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 Nov...

...protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant (YPet)...protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant (CyPet)...of a recombinant protein with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant. More...enough to get all the information you need. If you want to dissect an individual protein’s function under...highly controlled conditions in vitro , or, if you want to determine its structure using X-Ray crystallography...lactose/IPTG inducible vector for expression of a recombinant protein with an N-terminal 6x His tag and an ...

...pmScarlet-i_Giantin_C1 Golgi apparatus Giantin mScarlet-I Dorus Gadella 85049 pmScarlet-H_Giantin_C1 Golgi...apparatus Giantin mScarlet-H Dorus Gadella 85048 pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet ...mTurquoise2-Giantin Golgi apparatus Giantin mTurquoise2 Dorus Gadella 98880 mNeonGreen-Giantin Golgi apparatus...Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown 11908...protein is often tied to its function, so it's important to determine where your protein of interest resides..., if the marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein...Bruchez 44385 pLV-mitoGFP Mitochondria COX8 GFP Pantelis Tsoulfas 80000 pMito-miRFP703 Mitochondria COX8A...

...17798 pM-ErbB4CTF-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17799 pM-ErbB4CTF-PY3 mutant ERBB4 GAL4-BD ALS...delta-kinase-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD... Huntington's Nico Dantuma 23971 VCP(wt)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23972 VCP(R155H)-EGFP... HA CMV ALS Nico Dantuma 23973 VCP(A232E)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23974 VCP(DK0)-EGFP...pHA-deltaN335-Kin1A KIF5A HA CMV ALS Anthony Brown 31607 pKin1A KIF5A CMV ALS Anthony Brown 31717 pRK5M-ADAM10 ADAM10...Babu 128616 Anti-Kv1.1 K+ channel [K20/78R] KCNA1 CMV Episodic ataxia James Trimmer 128627 Anti-Kv4.3 K+ ...telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173759 pET-...

...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...monoclonal antibodies validated for neuroscience research. The plasmids encoding these antibodies are available...Initiative collection of plasmids, viral preps, and antibodies here. Collections...community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN Initiative...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...

...levels mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1 Cre expressed at low levels mutant TEF1 Yeast...levels mutant TEF1 Yeast Piper 60933 pPL5628_TEF1*-Cre_MET15 Cre expressed at low levels mutant TEF3 Yeast... ligand (e.g. tamoxifen) to activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated...alternative Cre recombinases that recognize specific mutant loxP sites, VloxP and SloxP sites respectively....Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding domain CMV Mammalian Green 12497...12497 p274 pCMV-CRE-M-AR(T) CREM fused to mutant AR ligand binding domain CMV Mammalian Green 12529 pBS...fused to HIV-TAT - promotes cellular uptake of recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc...