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Showing: 21 - 40 of 142 results
  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...
  2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...
  3. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...
  4. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy...open standard for microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-...
  5. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If...editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central...
  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic...in human stem cells. Mol Syst Biol. 2014 Nov 17;10(11):760. Weiss iPSCs & iPSC-NPCs Neurons Lentiviral ...into GABAergic Interneurons. Cells. 2020 Nov 10;9(11). pii: cells9112451. doi: 10.3390/cells9112451. Ottosson...
  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian cells without decreased efficiency...payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular...Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871...
  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...produce Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate...M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim...
  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central PMCID...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...
  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Ten-fold Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani...proteins in their ultrastructural context. Nat Commun 11:3850. https://doi.org/10.1038/s41467-020-17523-8 ...
  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, pp...Mammalian Cells.” ACS Synthetic Biology, vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp...
  12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...not include stem loop 3, and guide RNA nucleotides 11-20 are not resolved due to structural disorder. HNH...DNA target are essential for cleavage. Nucleotides 11-20 are disordered and don’t appear in the Figure ... editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739...
  13. Arf GTPase Family

    Type
    Collection
    ... Yohe ME, Kahn RA. Mol Biol Cell. 2019 May 15;30(11):1249-1271. doi: 10.1091/mbc.E18-12-0820. PubMed...
Showing: 21 - 40 of 142 results