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Showing: 21 - 40 of 151 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ... mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm...via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub...

  4. Typing CRISPR Systems

    Type
    Blog Post
    ...effector was developed, called Cas7-11. Despite being in Class 1, Cas7-11 is a single protein effector and...CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Makarova...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597(7878), 720–725. https://doi.org/10.1038...

  6. Plasmids for Stem Cell Research

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    Collection
    ...somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic...in human stem cells. Mol Syst Biol. 2014 Nov 17;10(11):760. Weiss iPSCs & iPSC-NPCs Neurons Lentiviral ...into GABAergic Interneurons. Cells. 2020 Nov 10;9(11). pii: cells9112451. doi: 10.3390/cells9112451. Ottosson...

  7. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy...open standard for microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-...

  8. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If...editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian cells without decreased efficiency...payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular...Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871...

  13. CRISPR Guide

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    Collection
    ...orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria meningitidis ...orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing genomic loci with fluorophores...optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927. PMID: 30377362 Casini, A., Olivieri, M...CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. PMID: 26411297 Marino, N. D., Pinilla-...using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L.,...double-stranded breaks. Biochemical Journal . 475 (11), 1955–1964. PMID: 29891532 Gaudelli, N. M., Komor...genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID: 30403660 Sanson, K. R., Hanna, ...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Ten-fold Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani...proteins in their ultrastructural context. Nat Commun 11:3850. https://doi.org/10.1038/s41467-020-17523-8 ...

  15. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...particle formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: ...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central PMCID...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...

  20. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...produce Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate...M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim...
Showing: 21 - 40 of 151 results