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  1. Hot Plasmids - January 2023

    Type
    Blog Post
    ... mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm...via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub...
  2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...
  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...
  4. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...
  5. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy...open standard for microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-...
  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic...in human stem cells. Mol Syst Biol. 2014 Nov 17;10(11):760. Weiss iPSCs & iPSC-NPCs Neurons Lentiviral ...into GABAergic Interneurons. Cells. 2020 Nov 10;9(11). pii: cells9112451. doi: 10.3390/cells9112451. Ottosson...
  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If...editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central...
  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian cells without decreased efficiency...payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular...Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871...
  9. CRISPR Guide

    Type
    Collection
    ...orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria meningitidis ...orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing genomic loci with fluorophores...optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927. PMID: 30377362 Casini, A., Olivieri, M...CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. PMID: 26411297 Marino, N. D., Pinilla-...using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L.,...double-stranded breaks. Biochemical Journal . 475 (11), 1955–1964. PMID: 29891532 Gaudelli, N. M., Komor...genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID: 30403660 Sanson, K. R., Hanna, ...
  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Ten-fold Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani...proteins in their ultrastructural context. Nat Commun 11:3850. https://doi.org/10.1038/s41467-020-17523-8 ...
  11. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...particle formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: ...
  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central PMCID...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...
  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...produce Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate...M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim...
Showing: 21 - 40 of 146 results