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  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these factors...need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time should be...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...dilution series of the standard and the minimum and maximum detection limits of the instrument being used to...specificity, especially as it approaches the minimum and maximum thresholds. If the measurements for the unknown...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ...plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits ...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...s illumination (see Figure 2). H describes the maximum signal strength, while W results from the time ... The resulting histograms is described through maximum current (H), the time the cell needed to pass through...

  8. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional allele...Selection of gRNAs for an experiment needs to balance maximizing on-target activity while minimizing off-target...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...population level, but each individual cell only has a maximum of one gene knocked out. In this genetically heterogeneous...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  10. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for....2% of studied sequences) (Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower ...

  12. AAVED

    Type
    Collection
    ...perform, recommended histology conditions, minimum/maximum incubation time after in vivo delivery, etc.) Spark...

  14. Plan Your Experiment

    Type
    Collection
    ... it is also important to consider factors that maximize cleavage of the desired target sequence or on-target...

  15. Luciferase Plasmids

    Type
    Collection
    ...self‐labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Conformationally...

  20. Hot Plasmids - October 2022

    Type
    Blog Post
    ...potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm and two-photon excitation ...

  23. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...need to add an additional 'G'. Q3: What is the maximum amount of DNA that can be inserted into the genome...

  30. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...special bacterial growth conditions in order to maximize the mutation rate. For example the Phage Assisted...

  38. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...orientations and components within the system to maximize production potential. For example, the best promoter...

  42. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...comparable expression of all components of Cascade for maximum efficiency of the process. Various labs have designed...

  45. MXS Chaining

    Type
    Blog Post
    ...Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 ...
Showing: 51 - 100 of 137 results