...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second...

...distribution funds our operation. Antibodies are glycoproteins that are part of the humoral immune system. ...against a target antigen. This contrasts with polyclonal antibodies, which are mixtures of monoclonal ...

...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

...as glyphosate, in water. He aims to leverage a glycine oxidase reaction to indirectly detect glyphosate...will co-express together with a plasmid encoding glycine oxidase. The goal is to determine potential water...

...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

...for antibody production have several drawbacks. Polyclonal antibodies produced in animals may be variable...potential for further engineering as compared to polyclonal or monoclonal antibodies. You can learn more ...

...single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly...higher plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give...

...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

...plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across both systems.  ...Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

...lower background signals than polyclonal antibodies. However, polyclonal antibodies are more sensitive...clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that are specific... antibodies are typically more expensive than polyclonal antibodies. Environmental factors, binding partners...available epitope on your protein of interest. Polyclonal antibodies are typically collected directly from...targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally stable...protein in any form it may be present in. However, polyclonals can vary significantly from lot to lot, as immune...consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be using a...

...encodes a single envelope protein called rabies glycoprotein (G). G-protein-coated RABV infects cells via...AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

... Ingredient Concentration or % Glycine or Glycine-HCl 25–200 mM SDS 0.1%–1% (w/v) ...

...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

...or supplying additional carbon sources such as glycerol or glucose (in moderation) may also serve to increase...fresh colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid...

...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

...Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...

...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

...such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine is very effective...

...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...mRNA  Strong yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very...

...avidin, is found in the egg white. Avidin is a glycoprotein that can bind four molecules of biotin. ...purified avidin (Kresege et al., 2004). Avidin is a glycoprotein that consists of four identical subunits that...

... a natural coenzyme, that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. ...various cell surface proteins that contain a monoglycine residue at the N terminus with a small peptide...

...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal...

...frequently alter their DNA by adding methyl groups, glycosyl groups, and other modifications to their bases... the interactions between bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion...

...-TMEM24 73093 GlycophorinA-CD4d3+4-bio 73094 GlycophorinB-CD4d3+4-bio 73095 GlycophorinE-CD4d3+4-bio 73096... 73096 GlycophorinC-CD4d3+4-bio 73097 CD99-CD4d3+4-bio 73098 CD44-isoform1-CD4d3+4-bio 73099 CR1-isoformB-CD4d3...

...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells Kotin 65214...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells Kotin 65215...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells Kotin 65216...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells Kotin 92307...

...MGC149868, hCS-L CSPG5 chondroitin sulfate proteoglycan 5 (neuroglycan C) MGC44034, NGC CTF1 cardiotrophin 1...MGC99678 GPHA2 glycoprotein hormone alpha 2 A2, GPA2, MGC126572, ZSIG51 GPHB5 glycoprotein hormone beta ...subunit ADTB3, ADTB3A, HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding ZA2G, ZAG B2M beta-2-microglobulin...osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen synthase kinase 3 beta - HRAS v-Ha-ras Harvey ...laevis) DAND4, MGC119894, MGC119895, MGC96951 CGA glycoprotein hormones, alpha polypeptide CG-ALPHA, FSHA, ...MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) GIF, GLIF, MMIF MLN motilin...gfg-1 OGFR opioid growth factor receptor - OGN osteoglycin DKFZp586P2421, OG, OIF, SLRR3A OPRD1 opioid receptor...

...pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 ...pOpen-T4 PGD T4 PGD (T4 pyrimidine DNA glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease ...

... single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...