...such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan genes ...bidirectional promoter, integrate at 11 specific chromosomal locations, and stably express the integrated ...are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for restoring...

...regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción...científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101: Una herramienta...

...instructions to navigate various lab processes.  Our most recent protocol video detailing Streaking Plates...you navigate Addgene’s vast online resources. Our most popular How-To video on “How to Order” details every...

...in Ghana and in other developing countries” and “Almost all cervical cancers are caused by persistent infection...get plasmids to the researchers who need them the most. Check back here to learn more about future awardees...

...tribulations of the modern PhD program. Don’t be fooled! Most senior graduate students are friendly and full of...University of California, Los Angeles. She enjoys chromosome biology and reading Ben Vincent's tweets.    ...

...readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A [email protected] if you are interested. Addgene's Most Popular Posts of 2014 How to Design Your gRNA...

...CRISPR plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably...from 231 countries. Guides and protocols were our most popular content, while our beloved blog received...

...dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the...year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...

...in the water of HR work. What do you think is the most important resource that Addgene provides the scientific...dogs and help screen dog adoption applications. My most recent foster dog was with us for two months before...

...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...of nonacademic science careers is that a team is almost always involved. Demonstrating your ability to ...

... the kit in December, GreenGate includes the six most frequently used elements in plant expression cassettes...generate multi-construct transgenic plants. In the most practical terms, what GreenGate can do is “save ...

...feedback from the team. Successful management can almost be boiled down to one, key concept: Creating a ... style.   Seeking feedback frequently One of the most important paths of information flow is the one that...

...how each one impacted cell viability (see figure). Most of the mutations were found to be “neutral,” having...gene is involved in a complex pathway. Some of the most heavily-studied genes in cancer research, like PIK3CA...

...organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone. They...requires an awful amount of skill,  time for which most researchers wouldn't have. They are motivated to...

...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells using zinc finger nucleases...

...communicate exactly what a site offers to their users, and most importantly, allow those users the ability to quickly...upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile, but occasionally you might...

... of information to fit into a single plasmid, so most first-generation binary vectors are quite large ...promoter. Originally isolated from the Mirabilis mosaic virus, the constitutively active M24 promoter has...

...advancing the field.   What is a plasmid? At their most basic level, plasmids are small circular pieces ...that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria, but also...

...display protein aggregation, it’s recommended for most imaging applications. What can you do with SunTag... experiments in their paper, and so can you! In almost any case you would use a traditional FP fusion,...

...Montgomery, or Louis Miller led these sessions. The most beneficial aspect of this course was breaking up...works.  4. Practice writing everyday Overall, the most important instruction the classes taught me was ...