...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument for controlling ...al, Nat Chem Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with...

...scientists starting their own labs get excited about making their first deposit to Addgene. We are also ...cutting edge practices and benefits and these are making a difference. We serve scientists around the globe...about being able to do good in the world. We are making an impact.  {% video_player "embed_player" overrideable...

...end, you’ll have a better understanding of what makes polyclonal antibodies unique and what experiments...together, all the B cell clones in the immune system make different isotypes of antibodies that recognize ...other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in many labs...

... always excited when depositors find new ways to make this workhorse protein even more useful! From FPs...retain normal neuronal function, this system may make optogenetics in specific cell populations even easier...applications, including optogenetics, and they should make it much easier to conduct functional studies in ...

...plasmid such as pX330. This cloning strategy not only makes it easy to create a single gRNA-expressing plasmid...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

...spatiotemporal control or an “on/off switch.” This makes them great tools for providing a global overview... of the bait protein will be biotinylated. This makes it’s impossible to distinguish proteins that directly... proteins. This extended incubation period could make it difficult to detect proteins with short half-...

...it also helps you to hone your writing skills and make a name for yourself. Pick a topic that is close ...’t you record a podcast or put some effort into making a video? Use your unique talent to communicate ...Since then, I have tried many things: photography, making videos, presenting in pubs and at science festivals...

...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing... if the mild is not effective. You may choose to make your own stripping solution using a recipe like ... Assess the stripping Now it’s time to check to make sure the antibodies from your first round of detection...

...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions ...posts, lesson plans, or information briefs for policymakers. The rapidly changing landscape of genetics ...

...Requiring an additional plasmid for transfection can make these optimization experiments a bit more challenging...experimental volumes accordingly. While you may need to make some final adjustments, this is generally a good...additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic determination of the packaging...

...vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are...vectors when used in experiments. Retroviral vectors make up a large toolbox used by researchers for (among...shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems, especially...

...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer...

...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

...meeting in person is often much more productive and makes it easier to build relationships. Nonetheless, the...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...such as leadership skills can only assist you and make you more qualified for future career opportunities...

... who keep changing their minds and have trouble making decisions. In my work as a thesis coach for graduate...be reviewed, bring them to the meeting. This will make it easy for your supervisor to support you. 3) Explain...articles on our blog Read Joanne Kamen's Post on What Makes a Good Mentor Resources on the Addgene Wesbite ...

...School of Medicine. In the Lindberg Lab we often make cell lines that overexpress genes of interest; more...Parents of infants will have no problem with this!) Make sure your colonies are well separated. For this,...taking both early- and late-arising clones, and making sure we always maintain a backup replicates of ...

... any features of the library backbone that would make the library more difficult to work with. For example...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ... reference protocols There are many decisions to make when developing your amplification protocol! We ...

...modulators regulate transcription and chimeric efforts make a precise edit or insertion via a chemical modifier...but it certainly suggests that Addgene depositors make CRISPR tools that fill needs in the community. The...centers around the impact of science, but our data makes it clear that it’s also a story of community, cooperation...

...m going to share what I’ve learned and hopefully make your decision a little easier if an industry postdoc... your goal is to enter academia, it is easier to make that transition from an academic setting—especially...company you choose. If publishing is important to you, make sure that your project will not involve proprietary...

...that commercially available cloning kits can often make the process much more streamlined, because they ...the reaction in favor of the desired assembly to make the process close to 100% efficient. Gateway cloning...Multiple inserts: There are some cloning methods that make working with multiple inserts a breeze. Among them...