Skip to main content
Addgene
Showing: 361 - 380 of 646 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity...

  3. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein...pulldown of a protein of interest, much like using ChIP for studying protein and DNA interactions. Unfortunately...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...approximately the length of your average PhD to achieve!) Each field has its own framework: a particular...images, and links to pay attention to. If you’re watching a video, you now have moving images, noises and...

  5. Working with Nuclear Receptors

    Type
    Blog Post
    ...Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...

  6. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the...

  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus (bovine), X. laevis (frog), D. rerio...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI:10.1038/...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...Mayo-Muñoz D, He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based...

  11. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of...Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional Resrouces...

  14. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...

  15. PhD Applications After COVID

    Type
    Blog Post
    ...research that aligns with your interests and goals.  Reaching Out to Potential PIs Even if you’re applying to...you determine if the program is the right fit. Reaching out to PIs also means doing plenty of mini-interviews...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

  19. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, ... Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T.,...

  20. Pooled Library Amplifications

    Type
    Blog Post
    ...decide what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere...weight of your library plasmids. As long as you achieve sufficient coverage from your transformation, any...
Showing: 361 - 380 of 646 results