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  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...expect to see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes...increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline that's ...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    ...translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not...handbooks for protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts ...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...chromosomal DNA. Chromosomal replication in bacteria is carefully regulated to ensure that it occurs at the appropriate...DNA replication in bacteria, so the process is carefully regulated by a mix of positive and negative regulators...

  8. Viral Production at Addgene

    Type
    Blog Post
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken offline and discarded. Confirmation of the transfer plasmid Addgene ...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing contributed to the update...PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics....

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibiotics is certainly a topic of great concern in healthcare today, but it doesn’t have to be some looming...resistance from the CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn...

  13. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get distracted...been building your creativity all along. In my own career, my team has used these techniques to optimize ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize the...approach appealing, but the disadvantages must be carefully considered before using. For example, the tag ...

  16. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by the name of the...reasonable time-frame. For these cases, some strains carry an additional measure of control such as the pLys...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...available resources and give yourself time to carefully review your options. If possible, try to select...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach...fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V...
Showing: 361 - 380 of 625 results