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Showing: 361 - 380 of 418 results
  1. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...
  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...
  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ... Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein...
  4. How to Be an Excellent Trainee

    Type
    Blog Post
    ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...
  5. Bioinformatics at Addgene

    Type
    Blog Post
    ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...
  6. MXS Chaining

    Type
    Blog Post
    ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...
  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...
  8. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the...
  9. Chemogenetics Guide

    Type
    Guide
    ...ligands. Specifically, R eceptors A ctivated S olely by S ynthetic L igands (RASSLs) based on κ-opiod ... JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, ...manipulation of ion channels are P harmacologically S elective A ctuator M odules (PSAMs, pronounced SAMs...specific small molecules termed P harmacologically S elective E ffector M olecules (PSEMs). PSAM domains...their activity in neurons PSAM Ion Pore Domain Ligand(s) Effect Outcome (in neurons) Reference PSAM4 Gly Varenicline...29351511 Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007). Evolving the lock to fit the key ...Haberman A, Graham J, Block J, Zhou W, Chen Y, Zhang S-C (2021). Human Stem Cell-Derived Neurons Repair Circuits...
  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked...
  11. Sequencing Primers

    Type
    Guide
    ...Invitrogen) S. cerevisiae GAL1 promoter, forward primer Gal10pro-F GGTGGTAATGCCATGTAATATG (Stratagene) S. cerevisiae... 5' GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer SP6 ATTTAGGTGACACTATAG... reverse primer GPDpro-F CGGTAGGTATTGATTGTAATTCTG S. cerevisiae GPD promoter, forward primer GW-3' GCATGATGACCACCGATATG...promoter, forward primer Nmt1-F GCAATGTGCAGCGAAACTAA S. pombe nmt1 promoter, forward primer OpIE2 Forward... 5' GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer pGP704-R AACAAGCCAGGGATGTAACG...
Showing: 361 - 380 of 418 results