...Scholars summer program. I was unsure whether or not I wanted to major in biochemistry. There, I had exchanged...has changed my life for the better, and I wouldn’t want it any other way. Toj Toj-Villagran is a rising ...

...reagents.  mAb3D atlas validated antibodies: A database of validated antibodies for mouse, human, and marmoset...

...researchers to select and generate physiologically relevant models. In complex diseases, it can aid in generation... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear...

...This series was written for selfish reasons: I wanted to learn about careers in science communication...summarizing mechanisms and applications that are relevant to things IDT does. I also work on our website...

...effector gene. Effector genes, any gene that you want to control the expression of using the SPARC system...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...

...different cellular responses. One of the biggest advantages of such a tool is being able to identify specific...screening technique. Lastly, the activity of both antagonists and inverse-agonists could be measured using ...

...two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made in the lab, rep and cap are...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

... et al calls this method acoustic collapse.  Importantly, acoustic collapse doesn’t affect bacterial viability... of ARG constructs with new acoustic phenotypes. Want to start using acoustic reporters for your research...

...chemistry degrees and provide advice to students who want to study chemistry. The society currently has 352...engaging with young scholars. We believe it’s very important to develop a network of fellow scientists that...

...simply put, I moved into this position because I want to help make the research enterprise work better...at Harvard, I recognized that I would ultimately want to go into science communication but thought that...

... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...focuses at Science on developmental neurobiology and plant sciences, viewed through the lens of development...

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... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

...were known to be diversity hotspots containing abundant biological dark matter and so the lab organized...application from the publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq...

...that produce useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously...mCherry and GFP expression to check for ABE’s. b) Quantification of base-editing efficiency using XMAS-TREE-...

...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that...

...to publication and acquisition of funding. If a grant committee is not convinced that your project is ...getting a much-needed break from your lab bench.  Want further information about shaping your career to...

...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...

...bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...

... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-...

...calling this “physical distancing” since it’s important to stay virtually connected with the people in...webinars. Share your work online. Maybe you’ve always wanted to start using social media professionally but ...

...while still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered...

...his love for science and later realizing that he wanted to continue doing research, Doench went on to apply...regulatory process. This early research is still relevant to his work at the Broad Institute today, where...

...circular DNA takes on several conformations the most abundant being: supercoiled, relaxed and nicked. If your...your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA will make it difficult...

...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...

... genome-wide screening,” Konermann said. “If you want to activate all 20,000 genes, it is not possible...genes that, when turned on, make melanoma cells resistant to treatment with a BRAF inhibitor. (The test ...

...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

...preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...

...journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship...

...for your audience. I ask myself that question constantly (and so should you!)  Amount of information and... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...

...of said containers, think carefully about how you want your software to treat those encoded numbers. Loosely...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

...Modifying gene expression Generally speaking, one may want to achieve either inhibition or activation of neurons...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol...

...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic locus...

...plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are...

...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure...to identify and reduce barriers for scientists wanting to share their tools with Addgene.  In our next...

...CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and... (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal subtypes of...

... Protocols DNA Quantification DNA Quantification You may also like... Addgene’s DNA Quantification Protocol. Protocols... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

...adapted for any antibiotic through the use of a selection curve to determine optimal antibiotic concentration...Learn how to add antibiotic to a plate after it's already been poured and solidified. Protocols...Streaking Bacteria on LB Agar Plate Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates...This protocol describes methodology for plating antibiotic over-agar for the selection of transformed E....E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate and... does not need to prepare separate batches of antibiotic-containing agar. This protocol will focus specifically...the protocol video below to learn how to spread antibiotic over-agar. Equipment Pipette tips for both pipetting...

...software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific...Collection Antibody Blog Posts Antibodies 101: ELISA Antibodies 101: The Four ELISAs and When to Use Them...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide Introduction This protocol...Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary antibody incubation...primary antibody and an isotype control antibody to the desired concentrations in the antibody dilution... with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary antibody for 2 ... a purified antigen to demonstrate the expected antibody dose response. Protocols...

...don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia...

...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

...all published videos in our Quick Tips playlist:  Want your quick tips even quicker? We’ll be sharing our...

...sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab...

...sweaters, Flannel Fridays, Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout...

... courses. The Intro to the Lab Bench series is meant to be an informative introduction for those who ...