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  1. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...competent cells and retransform. Bacteria on an LB agar plate can be stored at 4°C for a few weeks. However, if...stock unthaw! Streak the bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate...

  2. Colony Formation Titering Assay

    Type
    Protocol
    ...(μL) Volume of Virus Added to Plate (μL) Volume of Cells Added to Plate (μL) Final Viral Dilution 1:10...

  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex...reach 100% confluence (80–90% is ideal). Pass and plate the cells the day before the transfection. Thaw ...

  6. Ligation Independent Cloning

    Type
    Protocol
    ...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

  7. Pipetting Protocol

    Type
    Protocol
    ...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...
Showing: 341 - 349 of 349 results