... the company. I’d identified the ways to get the most out the resources we have, and we developed a great...But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices ....  Test: Our first official shoot went off with almost no hitches. The wheeled stand was a bit cumbersome...

...Microscopy (University of Amsterdam). GFP is the most popular, most widely used genetically encoded fluorescent...maturation to functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add...

... extracellular environment and the cytoskeleton. Most integrins have three conformational states: bent-closed...many function-blocking integrin antibodies exist, almost all bind outside the ligand-binding pocket, their...challenging the long-held belief that αVβ3 would be the most functionally important integrin on these cells. ...

...microtubule-cutting sword Microtubules are one of the most important components of the cytoskeleton. They provide...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...adhesion But what if you’re interested in that other most-important component of the cytoskeleton – actin?...

...previously appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications... A number of classic methods exist for this, the most common of which is the utilization of phosphomimetic...are available through Addgene, but we have worked most extensively worked with and optimized Sep-OTSλ and...

... Sanger sequencing of pooled samples showed that most of the HIV-1 genome had been excised, leaving only...rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy...therapeutic possibilities for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang...

... Equity and inclusion The final and possibly the most important panel of the symposium was on diversity...easily digestible/available. What I viewed as the most important take-away from this session was the idea...are making great progress on some of the world’s most profound questions, panelists and participants of...

...viruses that prey on bacteria) may be the most numerous and most diverse biological entities on our planet...recognition factors of phages) constitute one of the most popular classes of phage proteins to study. These...

...fact, we usually recommend testing more than one.  Most AAV serotypes have broad tropism (the ability of...promoters  These often-strong promoters offer the most flexibility for expression in a large number of ...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience...

... recognizing mentee Lucía Ramos (University of Valencia, Spain) as the most improved trajectory during...during the pilot, and Joaquín López (MIT, USA) as most outstanding mentor in this first program. Although ...

...phosphoproteins Protein phosphorylation is one of the most abundant forms of post-translational modifications...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...frequently, so visit our CRISPR pages often to find the most recently deposited tools, resources, protocols, ... popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies in Addgene's First...

... Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists I’ve met began doing research ...epidemiology, public health, and more.     A few of the most common roles that you’ll see in a lab. Position...dedicate your time and energy to. How can I make the most of my experience in the lab? Ask your questions—...

...ensuring the PAMs remain on the chromosome after DSB formation, was most efficient (Farboud et al., 2019...Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based...

... used. Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector system is...this, you can use the transfection reagent you are most comfortable with. If you don’t have any experience...depends on the type of cell you would like to infect. Most of the protocols you will find will recommend using...

...development, the history of optogenetics begins almost 50 years ago with the discovery of microbial rhodopsins...electrical signal down the axon (Lodish et al., 2000). Most neurons in the brain are not naturally light-sensitive...system due to their relatively stable extra-chromosomally long-term expression and low ability to induce...

...where excitation and emission are the most efficient (i.e., happen most of the time). Figure 1 shows a graph...molecule), their bleed is so strong that these spectra almost overlap. Hence, they should not be used within ...

...the number of cells needed is lower, and perhaps most important, targeting all genes doesn’t necessarily...group works on immune cells in different contexts. Most of the immune cells we study are migratory, and ...candidate genes. Green Listed, as of now, contains most of the full genome knockout human and mouse libraries...

...the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com....conformation frequently (Osuka et al., 2018), but it’s most often shown in an “open” state, with the NUC and...region is disordered, Cas9 binds DNA very weakly, most likely through unstructured electrostatic interactions...

....  The information presented may not reflect the most current legal developments.  Further, this post ... to their areas of interest. Additionally, since most universities and non-profit organizations do not...applicable to scientists). High Salary –not relevant to most postdocs. Commercial successes in the performing...

...alternative approach is RNA interference (RNAi). The most common method is transfection of short interfering...protein), which has been designated CD365 in the most recent HLDA workshop (HLDA10), as an example. For...cell lines using these two antibodies. This is the most usual level of evidence supplied to support the ...