Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 771 results for: Dos

Showing: 341 - 360 of 771 results

  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ..., but from what was necessary in order for you to do your research. For example, did you help put together...extracurricular activities – did you volunteer to do science outreach and teach children about science...

  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things ...of great projects as ‘impossible' or useless.” How do you run your lab differently or the same as your ...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle.... AAVs are therefore non-pathogenic and generally do not trigger a strong immune response. These features... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...vectors present low oncogenic potential because they do not integrate in the human chromosome, which reduces...

  4. 2022 Depositor Week

    Type
    Blog Post
    ... Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...observe your cells on a flow plot, you might have to do some testing or ask experienced colleagues. ...warning message at the top, as our cells of interest do not lie beyond the dimensions displayed in the plot...4). As H and A scale in proportion (while H and W do not; see Figure 2), single cell events will show ...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...and vocabulary for this skill. What this post may do is help you realize whether or not you have a natural...t mean you have to like conflict. Very few people do, and it’s best to be wary of anyone who is actually...there is a potential for long-term gains when you can do it. If Party X proposes solution A, and Party Y proposes...

  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...increase therapeutic efficacy (Ahmed et al., 2012). How do you generate an scFv?  scFv sequences have traditionally...antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s possible to produce...scFvs are even used to screen for new antibodies. To do this, antibody variable domain sequences are expressed...

  9. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

  12. Antibodies 101: ChIP

    Type
    Blog Post
    ...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

  15. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...

  17. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...

  18. The Twelve Days of Antibodies

    Type
    Blog Post
    ... to our blog and social media. This year, we're doing so through the twelve days of antibodies. We hope...

  19. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...since most universities and non-profit organizations do not have the April 1 H-1B queue process or quota ... the performing arts- not relevant at all. Please do not include the fact that you are a classically trained...self-petition. You need the support of your institute to do this one. Universities will usually only support ...who could be beneficial during a career search. Doing these simple things will make it easier for you ...

  20. Plan Your Experiment

    Type
    Guide
    ... model a specific disease or process of interest. Do you want to: Generate complete and permanent loss...target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, ...a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before choosing a validation...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described...
Showing: 341 - 360 of 771 results