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Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

The HA frankenbody combines optimized elements of an antibody probe with a fluorescent protein and can be used to easily visualize single molecules and newly formed proteins. 

Resources to help improve precision genome editing through homology directed repair in experiments with CRISPR nucleases.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Advice on starting a lab from three PIs who have successfully built their own academic labs.

Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.

Browse Addgene's collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs.

Discover our AAV Packaged on Request service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such as titering, purity assessment, sterility testing, and viral genome sequencing.

Browse AAV transfer, packaging, and helper plasmids in Addgene's repository.

Thank you to all the researchers, labs, and scientists who have deposited with Addgene! Check out all the cool events we have planned to celebrate this week.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

Learning to gate in a flow cytometry experiment can be difficult. Read our Intro to Gating post to help you get started!

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

An introduction to conflict resolution, identifying types of conflict and strategies for solving them.

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

Flow cytometry can require some extra controls, described in this blog post!

PhD student Emma Harcourt explains what it's like to do research in the field of science communication.

A basic overview of chromatic immunoprecipitation (ChIP)