...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things ...of great projects as ‘impossible' or useless.” How do you run your lab differently or the same as your ...

... Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for...

...increase therapeutic efficacy (Ahmed et al., 2012). How do you generate an scFv?  scFv sequences have traditionally...antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s possible to produce...scFvs are even used to screen for new antibodies. To do this, antibody variable domain sequences are expressed...

...observe your cells on a flow plot, you might have to do some testing or ask experienced colleagues. ...warning message at the top, as our cells of interest do not lie beyond the dimensions displayed in the plot...4). As H and A scale in proportion (while H and W do not; see Figure 2), single cell events will show ...

...the chance of off-target effects, you still need to do your due diligence to ensure they are indeed a non-issue...off-targets don’t mess with your results when they do arise. Happy CRISPR’ing! References and Resources...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9...

...and vocabulary for this skill. What this post may do is help you realize whether or not you have a natural...t mean you have to like conflict. Very few people do, and it’s best to be wary of anyone who is actually...there is a potential for long-term gains when you can do it. If Party X proposes solution A, and Party Y proposes...

...unbound) population. The benefit of beads is that they do not require cells from your experiment, and they ...prefers to use them to gate our populations, but others do not. Some journals require isotype controls, while...

...process is very similar for both, though tissue samples do require an extra homogenization step. For this blog...courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked...

... this given that the brain controls everything we do, but I was quite impressed by how neuroscientists...to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog Advanced...

...elute YFP, you turn on the blue lights. Which option do you choose?  Option #2 may sound like fiction, but...monomeric variable heavy chain antibody domain.  How do these components interact? When fused together to...

...we have found ourselves adapting to a new way of doing things. These new processes have been instrumental... what we’re here for and we’re glad to be able to do so during this critical time. Visit our COVID-19 ...

...similarity. REPLACR-mutagenesis: Letting bacteria do the work If you’ve conducted SDM using some of these...on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...

...? Blue-white selection is a widely used method to do just that! (To read about other screening strategies...with this method to ensure that positive colonies do have the plasmid containing the lethal gene. An example...

... all driven by personal connections What can you do to get the most from the time and money you are spending...about how you want to introduce yourself and what you do. You can probably use some scientific terms when ...

...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...

...coupled receptors? Read the GPCRs: How Do They Work and How Do We Study Them? blog! Studies of GPCRs led...are engineered to respond to small molecules that do not affect endogenous signalling, therefore allowing...DCZ seem to have minimal off-target activity, and do not seem to have the same back-metabolism issues ... consider: Chemogenetic activation or inhibition. Do you want to turn ON or turn OFF neurons in your experiment...high potency, which is potentially important for dosing in animal studies, made them attractive targets...

...transfer plasmids do not have selectable markers conferring resistance to an antibiotic, but do encode another...pRRE and pRSV-Rev . 3rd generation packaging systems DO NOT express tat. Third generation lentiviral systems...Addgene's Plasmid Reference and Protocol Guide . How do I clone my insert into a transfer vector with no ...system in total). In addition, 3rd generation systems do not use the HIV protein tat in order to produce full...

...since most universities and non-profit organizations do not have the April 1 H-1B queue process or quota ... the performing arts- not relevant at all. Please do not include the fact that you are a classically trained...self-petition. You need the support of your institute to do this one. Universities will usually only support ...who could be beneficial during a career search. Doing these simple things will make it easier for you ...

...areas of research. We welcome your comments... Where do you think these technologies are going? What is the...

...promoter region.   Ok, I have a Cas9! What do i do next? Designing or selecting a sgRNA Addgene may...