...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can...

...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...

...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat...

...you volunteer to do science outreach and teach children about science? Or perhaps you have presented in...specifically to assist graduate students in job searching. Start your career planning early, start now Don't...

... 3) produces bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results...however, you may need to run the gel for longer to achieve good separation of larger DNA fragments. I hope...

...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc...

...approaches. However, with each new technical achievement, the complexity of the device increases, as does...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

...tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...

... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...

...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell.... 7th edition (2000). Meibom, Karin L., et al. "Chitin induces natural competence in Vibrio cholerae."...

...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

... Those two things, in my opinion, had not been achieved.” That is, until now. Enter the SAM pooled library...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target effects...

...using a sequencing machine that recognizes the different fluorescent labels. The machine detects which fluorescently...replication (by bacteria) by recruiting DNA replication machinery. The ori is critical for the ability of the plasmid... in the bacteria by bacterial transcriptional machinery. Multiple Cloning Site (MCS) Short segment of ...promoter is designed to recruit transcriptional machinery from a particular organism or group of organisms... direct cell-specific expression, which can be achieved by a tissue-specific promoter (e.g., a liver-specific... when sequencing is not 100% efficient and the machine cannot distinguish between the 4 labelled nucleotides...

... of its biggest advantages. Specificity can be achieved using specialized promoters, Flp or Cre dependent...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

...the University of Illinois and the University of Michigan - initially cancelled their plans, but have since...plans and budgets working around issues such as childcare using money from their increased salaries. In ...

...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity...

... making fluorescent protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent...Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom PH, Oler...

...including difficult-to-manipulate primary cells, achieving knockdown within 1-2 hr of reagent delivery. TRIM-Away... gene expression regimes that cannot be easily achieved with classical gene overexpression and knockout...