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  1. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical copy on hand as... to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing a Lab...

  2. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

  9. Working with Nuclear Receptors

    Type
    Blog Post
    ..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...

  10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...not yet fully understood, so it is important to carefully select the target locations of knock-in studies...systems offer a number of different options. By carefully considering your ncRNA of interest, you should...

  11. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...for follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team...Sciences at Arizona State University. His group carries out research in synthetic biology, nanomaterials...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the molecular biology to learning how to grow and care for the worms. As an example of one of the problems...cheap using equipment that would otherwise be discarded. For instance, the centrifuge in the picture had...

  13. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...can be transfected with your transfer plasmid, carrying your gene of interest, and the two helper plasmids...undergoing titering, to remove residual plasmid DNA carried over from the production process. For more details...

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...this protein compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated...with this encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve ...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...15%, 25%, 40%, and 60% iodixanol solutions are carefully layered and then the viral suspension generated...digested to remove any residual AAV plasmid that was carried over from the AAV production process. Either SYBR...

  19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...

  20. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to the light, where they appear...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...
Showing: 341 - 360 of 625 results