Addgene

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Neurodegeneration Research Collection

Type

Collection

...and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter...
CRISPR Plasmids - Tagging

Type

Collection

...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...
15 Hot Plasmids from 2017

Type

Blog Post

...their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 ...the popular type II CRISPR systems, which include S. pyogenes (Spy) Cas9, been identified. In Rauch, et...
Pooled Library Amplifications

Type

Blog Post

...and how many cells to use for your transformation(s). The amount of competent cells you need total and...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

...References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding...
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

...in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)...
Summer SciComm Series: Modes of Communication

Type

Blog Post

...following four questions to figure out which method(s) work best for you, your audience, and your science...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...
Chemogenetics Plasmids

Type

Collection

...D esigner D rugs (DREADDs) and P harmacologically S elective A ctuator M odules (PSAMs). DREADDs are the...
Great Results Start with Great Standard Curves

Type

Blog Post

... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...
New CRISPR Tools: Cas7-11 and PASTE

Type

Blog Post

...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...
Getting to Know Expansion Microscopy

Type

Blog Post

...992. https://doi.org/10.1038/nbt.3625 Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...
Transferable Skills Guide: Career Planning Resources

Type

Blog Post

...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...
DNA Purification Without a Kit

Type

Blog Post

... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

Showing: 341 - 360 of 418 results

Results per page:

Neurodegeneration Research Collection

CRISPR Plasmids - Tagging

15 Hot Plasmids from 2017

Pooled Library Amplifications

Twenty Years of Addgene Sharing: CRISPR

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Summer SciComm Series: Modes of Communication

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Google Forums Round Up: First Impressions of NgAgo

Your Lentiviral Plasmid FAQs Answered

Chemogenetics Plasmids

Great Results Start with Great Standard Curves

New CRISPR Tools: Cas7-11 and PASTE

Viral Vectors 101: Preparing Pooled Libraries

Getting to Know Expansion Microscopy

Transferable Skills Guide: Career Planning Resources

AAVs CREATed for Gene Delivery to the CNS and PNS

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

DNA Purification Without a Kit

New Tools Enable CRISPRa for Neuroscience Applications

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