...the Myc tag to the world, it has become one of the most commonly used tags in molecular biology. And the...we all really using the same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody...design experiments. It turns out that even with the most popular antibodies, context is key.    References...

...production site to its final destination. As an example, most secreted proteins are produced in the endoplasmid... selected FPs need to be sufficiently separated, most commonly FPs with red and green wavelengths, respectively...microscopes the detection limit is 200 nm. The size of most proteins is below 10 nm and therefore colocalization...

...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...an extra wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰!...recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes ...

...response. At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and...limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot... is back, better and safer. It is now one of the most active and exciting areas of science. This is hopefully...

... we’ll break down the mechanism of action of the most common antibiotic resistance genes and which applications...bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin...?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies? Ampicillin Fair...

...the world encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier ...placing orders per day across regions, with Europe most impacted and China least impacted. The inferred ... China COVID-19 date range is, they could be the most impacted or least impacted. These results highlight...

...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with the...of research impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral expression...

....com.   The problem of antibiotic resistance Most antibiotics target one of three essential processes...are easy to see. As discussed in this blog post, most bacteria have poor non-homologous end joining machinery...specificity - rather than targeting a process essential to most bacteria, custom CRISPR antimicrobials can target...

...engineering tool. It is easy to use, functional in most species, and has many applications. That said, SpCas9...terms of structure, Cas13a shares no homology to the most commonly used CRISPR enzymes - Cas13a contains two...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any ...

...the fly community. Not surprising, this is where most of our conference room names originated from. Let...that contain SHH, you can find them all here! The most popular SHH plasmid however was deposited by none...insert specific pieces of DNA into vertebrate chromosomes via a cut and paste mechanism. Scientists reconstructed...

... just creating, which for many scientists is the most enjoyable aspect of their chosen career. You can...’t know what it’s like to be properly onboarded. Most work environments outside of academia provide a ...skills you pick up wherever you work, and you’ve almost certainly developed some in the lab whether you...

... exist for reducing Cas9 off-target effects, but most studies have focused on the off-target cutting of...not associated with ricin regulation, including almost all genes involved in the production of a cell ...fail to identify some known ricin-regulators, but most of these genes are also essential for growth and...

...much easier to map these neuronal networks. For almost three decades, recombinant Adeno-associated virus...IHC) experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis... microscopes, but they can also be used with the most cutting-edge imaging technologies such as array ...

...fluorescent visualization techniques for decades, most notably for fluorescent in situ hybridization (FISH...readout may render this approach less sensitive in most applications. Learn About Fluorescent Biosensors...Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. ...

...block of time was. Eventually, I learned that I was most productive in short bursts (even twenty minutes ...minutes was enough to make some progress). For most researchers, it’s not always possible to pull a Thoreau ...useful data Typically lists and pictures are the most useful parts of reviews. These could be in the form...

...markers are set up sequentially, from broadest to most specific marker, and are represented as a series...quadrant will often be too clean for real-world data. Most plots will have a shape, like a box, ellipse, or...  As the gates progress from broadest markers to most specific, the first step is to separate cells from...

...your best to show up as your most prepared, can-do self and make the most of it! So, what can you do ...dynamic and set yourself up for success! Getting the most out of the training experience In research there...

... is reinforcing, the animal will prefer to spend most of its time in the chamber in which it receives ...conversely, if the circuit is aversive, it will spend most of its times in the opposite chamber) [2]. Laser...standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species. However...

...side of things that have the most utility; i.e. those things that will be most useful for achieving your ...finish any additional work you might need to do. In most cases, I would recommend the second option for the...

...types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...associated with dementia, such as APP and PSEN1, in most cases the intended HDR events were corrupted by ...’t get what we wanted. The Indels were gone from most HDR reads that we studied by deep sequencing, but...