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  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris ...10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved broad-host-range...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional...Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens. Cell Systems...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Multiple uses: From multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing...autofluorescence, low scattering, and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged...

  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...to understand. (1-2 sentences). For example: Distinguishing correctly folded and misfolded proteins is ...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...of a problem when the excitation wavelength is shifted to longer wavelengths. Therefore, the use of bright...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered...effective for introducing non-sequence specific frameshift mutations through aberrant double strand break...

  8. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...it. The other half of each day you can spend accomplishing small tasks (like... preparing plasmids to send... Send out as many emails as possible While accomplishing any one of these things won’t mark an end to...

  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...develop a biosensor for oxytocin, Daisuke Ino and Hiroshi Hibino and colleagues devised a “Modular fluorescence...fluorescent biosensors for monitoring RTK activation by shift in cellular localization. Image adapted from Farahani...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay...Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein...

  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...approximately 1 in 5000 males. DMD is caused primarily by frameshift mutations in dystrophin, a protein essential ...regions, so low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) ...

  12. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...experimental condition you could look for a slight size shift in the protein band before and after treatment.  ...0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender ...

  13. A Control for All Seasons

    Type
    Blog Post
    ... western blot and look for the molecular weight shifts indicative of tau phosphorylation. By including... standard (P) to check for tau molecular weight shifts. A negative control is a sample or group that...

  16. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...member who knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands...critical features based on my own experience: Leadership Few things are more frustrating than being on...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...channels. In a typical assay a user will compare the shifting intensity of events. Since intensity positively...positively correlates with expression, the further the shift of a cell from the negative control, the higher ...

  18. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how...

  19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated...PMID:16550175. 6.Ando, Ryoko, Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling...

  20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure...PubMed Central PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements...
Showing: 321 - 340 of 532 results