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Showing: 321 - 340 of 557 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... E. coli strains E. coli are gram-negative, rod shaped bacteria that were named after Dr. Theodor Escherich...strain is a bit more convoluted due to researchers sharing and renaming samples throughout history. It was...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...doi.org/10.1016/j.omtm.2022.07.010 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...j.neuron.2016.09.021 Walters, R. W., Yi, S. M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. ...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...binding pocket of an engineered receptor that change shape with light exposure to activate them. PORTLs use... Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H....

  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ....org/10.1038/s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...blocking for 1 h at room temperature with gentle shaking, gently wick off excess liquid from each membrane...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...how science can be accelerated immensely when we share knowledge and reagents. I have not intentionally...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... also known as immunoglobulins, are ~150 kDa, Y-shaped proteins that are both a natural part of the immune... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies...

  12. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ... focus). This means that the images will appear sharp without interference from blurry out-of-focus light... lightsheet that projects a number of thin cone-shaped beams into the sample. These beams interfere to...

  14. Deep Dive: qPCR

    Type
    Blog Post
    ...and reliable qPCR data, you’ll of course want to share it so others can provide you with the highest form....2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...

  16. New Western Blot Protocol Video!

    Type
    Blog Post
    ... Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.    You can check...

  18. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...isn't your platform, all of our videos will also be shared on our @addgene Instagram account, or you can watch...

  19. 2022 Depositor Week

    Type
    Blog Post
    ...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  20. CRISPR Guide Update

    Type
    Blog Post
    ...With this update, we have added new sections to share and describe some incredible new CRISPR technologies...
Showing: 321 - 340 of 557 results