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We narrowed to 768 results for: Dos

Showing: 321 - 340 of 768 results

  1. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...at a peer audience.  Note that lay people can and do consume scientific communication (it is, after all...moves from layperson to expert) and peers can and do consume science communication.  Formal education ...

  2. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript ...introduction, etc. so as to thoroughly explain the do’s and don’ts of that week’s piece of a manuscript....

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ... single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should...

  4. p53 Pathway

    Type
    Collection
    ...Biology. 2010 Feb;2(2):a001107. PMC PMID: 20182618 . Do you have suggestions for other plasmids that should...

  5. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... FRET) with pmVenus(L68V)-mTurquoise2 Back to Top Do you have suggestions for other plasmids that should...

  6. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... that is right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid...midiprep, etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different...starting culture conditions rather than your kit.   Do you want eco-friendly options? It’s no secret that...  While budget is an important consideration, you do want to make sure you’re getting the best bang for...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...chemically coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1.... answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of...Chtanova et al. 2008, Kamenyeva et al. 2015)? How do blood monocytes patrol blood vessels during immune...Auffray et al. 2007, Finsterbusch et al. 2016)? How do dendritic cells migrate to lymph nodes to activate...

  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors...supporting data and community engagement required to do this drove Addgene to develop new systems, new partnerships...resources, and collaborations, and will continue to do so, in the hopes that these findings can help others...

  9. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...site-directed knock-in. We’ll also discuss what to do if your editing efficiency isn’t as great as you ...insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts is Tracking...base pairs modified? In these cases, it’s best to do some planning in the design phase of your experiment...

  10. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...guides as negative controls. Non-targeting guides do not target any site in the genome. This means that...confound the results of high-throughput CRISPR screens. Do short guides (17-18 bp) have less off-target cutting...have fewer off-target effects than long guides, and do not have a major reduction in on-target efficacy....

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as...economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely...Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...expression levels for most purposes, but what do you do when you’ve tried a common strain and don’t get...popular commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from...

  13. An Addgene Summer Internship

    Type
    Blog Post
    ...physics and sustainable design while allowing me to do my internship in the daytime.  Getting into the office...be heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions ...

  14. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...to infect with the virus. Then the viral particles do the rest! Downstream expression or integration resulting...biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated ...

  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Rooms to divide the audience into small sessions and do a quick break to chat. This can be done randomly ...from academia to industry”. To date, aside from doing science, he has assisted many researchers in gaining...

  18. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...ends results in the production of blue colonies that do not need to be picked and sequenced for potential...PCR, restriction digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need...

  19. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ..., but from what was necessary in order for you to do your research. For example, did you help put together...extracurricular activities – did you volunteer to do science outreach and teach children about science...

  20. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things ...of great projects as ‘impossible' or useless.” How do you run your lab differently or the same as your ...
Showing: 321 - 340 of 768 results