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We narrowed to 773 results for: Dos

Showing: 321 - 340 of 773 results

  1. AAV Packaged on Request

    Type
    Collection
    ...must place your order to begin production. You can do this whenever you are ready. Payment information ...

  2. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... that is right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid...midiprep, etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different...starting culture conditions rather than your kit.   Do you want eco-friendly options? It’s no secret that...  While budget is an important consideration, you do want to make sure you’re getting the best bang for...

  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...chemically coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1.... answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of...Chtanova et al. 2008, Kamenyeva et al. 2015)? How do blood monocytes patrol blood vessels during immune...Auffray et al. 2007, Finsterbusch et al. 2016)? How do dendritic cells migrate to lymph nodes to activate...

  4. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...site-directed knock-in. We’ll also discuss what to do if your editing efficiency isn’t as great as you ...insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts is Tracking...base pairs modified? In these cases, it’s best to do some planning in the design phase of your experiment...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors...supporting data and community engagement required to do this drove Addgene to develop new systems, new partnerships...resources, and collaborations, and will continue to do so, in the hopes that these findings can help others...

  6. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...guides as negative controls. Non-targeting guides do not target any site in the genome. This means that...confound the results of high-throughput CRISPR screens. Do short guides (17-18 bp) have less off-target cutting...have fewer off-target effects than long guides, and do not have a major reduction in on-target efficacy....

  7. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as...economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely...Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors...

  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...expression levels for most purposes, but what do you do when you’ve tried a common strain and don’t get...popular commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple Cas9s to be used...have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors...

  11. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Rooms to divide the audience into small sessions and do a quick break to chat. This can be done randomly ...from academia to industry”. To date, aside from doing science, he has assisted many researchers in gaining...

  13. An Addgene Summer Internship

    Type
    Blog Post
    ...physics and sustainable design while allowing me to do my internship in the daytime.  Getting into the office...be heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions ...

  14. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...to infect with the virus. Then the viral particles do the rest! Downstream expression or integration resulting...biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated ...

  15. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...ends results in the production of blue colonies that do not need to be picked and sequenced for potential...PCR, restriction digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need...

  16. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ..., but from what was necessary in order for you to do your research. For example, did you help put together...extracurricular activities – did you volunteer to do science outreach and teach children about science...

  17. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things ...of great projects as ‘impossible' or useless.” How do you run your lab differently or the same as your ...

  18. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle.... AAVs are therefore non-pathogenic and generally do not trigger a strong immune response. These features... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...vectors present low oncogenic potential because they do not integrate in the human chromosome, which reduces...

  19. 2022 Depositor Week

    Type
    Blog Post
    ... Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for...

  20. Arf GTPase Family

    Type
    Collection
    ...opens in a new window) Last reviewed on: 18 Jun 2025 Do you have suggestions for other plasmids that should...
Showing: 321 - 340 of 773 results