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1. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-...

2. Hot Plasmids - February 2022

   Type

   Blog Post

   ...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a...

3. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   ...This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...

5. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...input. Usually that function is in the shape of an S; there is a narrow threshold like a switch that turns...

6. Neurodegeneration Research Collection

   Type

   Collection

   ...and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter...

7. CRISPR Plasmids - Tagging

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   Collection

   ...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...

8. Antibodies 101: Fab Fragments

   Type

   Blog Post

   ....1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi ...

9. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   ...Together these two experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent...

10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    ... to do once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide...

11. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    ...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used...

13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure...

14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...

15. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...audience is expending to pay attention to the mode(s) of communication you choose.  Audience knowledge ...

16. What's New in CRISPR - November 2021

    Type

    Blog Post

    ...By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large...

17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...References Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability...

18. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    ...Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective...

19. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    ...that ultimately builds up to an overall conclusion(s). In some articles, the data are presented to build...

20. A Look at Addgene's QC Process

    Type

    Blog Post

    ... the de novo NGS result to the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps ...