We narrowed to 441 results for: %s

1. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   ...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

2. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   ...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts...

3. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   ...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...

4. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   ... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...

5. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-...

6. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   ...This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   ...input. Usually that function is in the shape of an S; there is a narrow threshold like a switch that turns...

9. Neurodegeneration Research Collection

   Type

   Collection

   ...and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter...

10. CRISPR Plasmids - Tagging

    Type

    Collection

    ...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...

11. Antibodies 101: Fab Fragments

    Type

    Blog Post

    ....1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi ...

12. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ... to do once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide...

13. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...audience is expending to pay attention to the mode(s) of communication you choose.  Audience knowledge ...

14. What's New in CRISPR - November 2021

    Type

    Blog Post

    ...By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large...

15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...References Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability...

16. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...Together these two experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent...

17. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ... guide AAV Viral Preps References  Chen, Y., Hu, S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    ...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas...

19. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used...

20. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure...