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...global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of...

...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ...

...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

...resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect...

...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly...

...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ...

...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...

...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ...

...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using...

...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...

...complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...

...DNA repair.  A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...

...is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for...

.... and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has ...

...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the...

...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...

...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...

...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those...

...edition.  References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ...

...come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General...