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Highlights of CRISPR impact in the drug discovery pipeline prior to clinical advancement.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

Tips for being more environmentally friendly in a life science research lab.

Struggling to get started with designing your first flow panel? This blog walks you through what you need to know and an example panel design to help you get started!

Learn about fluorescent protein techniques you can use to monitor cellular trafficking throughout an organism.

An introduction to Gram negative and positive bacteria - what are they, how can you tell the difference, and where did this terminology come from?

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

The HA frankenbody combines optimized elements of an antibody probe with a fluorescent protein and can be used to easily visualize single molecules and newly formed proteins. 

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including calcium sensors, GABA sensors, voltage sensors, and control AAV.

Learn how you can use a plasmid from the Rex lab at Vanderbilt to easily and selectively biotinylate your protein of interest.

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

Learn about what it takes to start your own business in the biotechnology field.

A basic overview of chromatic immunoprecipitation (ChIP)

Learn about new and upcoming viral vectors from Addgene including our new trial size AAVs for serotype testing, calcium and GABA sensors, and more!

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. Read more to learn about the recipients of the Addgene-Seeding Labs plasmid grant this year.

The purpose of this article is to explain what Unique Persistent Identifiers are, why they’re important, and to announce that Addgene now supports two different formats of plasmid identifiers and how they should be used.