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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 358 results for: plat

Showing: 301 - 320 of 358 results

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...that can be combined with your gRNA and repair templates of choice though modular cloning steps. The Voytas...

  19. Molecular Biology Reference

    Type
    Guide
    ...common is the Illumina NGS platform for short-read sequencing. This is the platform Addgene primarily uses...fragments of DNA, or templates, are spatially separated. These attached DNA templates are then amplified...again, producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. For more information on growing bacterial cultures...binds to the single strand of DNA. Using this as a template, the polymerase migrates down the DNA, adding ...nucleotides (dNTPs), a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi-parallel sequencing platform that can generate sequencing data for up to 600...

  20. CRISPR Guide

    Type
    Guide
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...binding site (PBS) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired...driving mismatch repair using the edited strand as a template PE4/5 — improved PE2/PE3 to inhibit mismatch repair...
Showing: 301 - 320 of 358 results