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We narrowed to 349 results for: plat

Showing: 301 - 320 of 349 results

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...that can be combined with your gRNA and repair templates of choice though modular cloning steps. The Voytas...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have...

  10. Molecular Biology Reference

    Type
    Guide
    ...common is the Illumina NGS platform for short-read sequencing. This is the platform Addgene primarily uses...fragments of DNA, or templates, are spatially separated. These attached DNA templates are then amplified...again, producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. For more information on growing bacterial cultures...binds to the single strand of DNA. Using this as a template, the polymerase migrates down the DNA, adding ...nucleotides (dNTPs), a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi-parallel sequencing platform that can generate sequencing data for up to 600...

  11. CRISPR Guide

    Type
    Guide
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...binding site (PBS) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired...driving mismatch repair using the edited strand as a template PE4/5 — improved PE2/PE3 to inhibit mismatch repair...

  12. Plan Your Experiment

    Type
    Guide
    ... donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing...Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle on your gRNA, ...Editing : pegRNAs must serve as both a guide and a template. In general, edits must be downstream (3’) of ...edit site yield higher editing efficiencies. The template sequences can also have a large impact on editing... desired edit. Even if you provided a homology template, a large percentage of DNA breaks will be repaired...

  13. Antibody Guide

    Type
    Guide
    ... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

  14. Adenovirus Guide

    Type
    Guide
    ...also made them the most widely used viral vector platform for vaccine design against a diversity of viruses...AdMax™ is another well used rAdV vector production platform, which like AdEasy™, relies on the homologous ...Tai, P. W. L., & Gao, G. (2021). Viral vector platforms within the gene therapy landscape . Signal Transduction...Curiel, D. T. (2021). Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic . NPJ Vaccines, 6 (...

  15. Promoters

    Type
    Guide
    ...produced. The antisense strand is referred to as the template strand or noncoding strand, as this is the strand...allow for the initiation of transcription of the template strand. The TATA box is a DNA sequence (TATAAA... is the RNA responsible for providing a stable template for the translation of a protein. RNA polymerase...

  16. Modular Cloning Guide

    Type
    Guide
    ...Expression, Mammalian Expression Stephan Gruber Platform for rapid assembly of expression vectors for multi-subunit...TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome Engineering, TALEN Takashi...Yamamoto Plasmids for construction of highly-active Platinum TALENs for use in a wide variety of target organisms...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...kidney 293T (HEK293T) cells are the most common platform to produce AAV vectors. Additional production ...infection, forming a double-stranded DNA (dsDNA) template. This eliminates the requirement for host cell...

  18. Optogenetics Guide

    Type
    Guide
    ...High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from...

  19. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... an automated ELISA plate washer. Seal the plate. Place the plate on a microplate shaker set at 400 rpm...use an automated ELISA plate washer. Seal the plate Place the plate on a microplate shaker set at 400 rpm... the plate with a plate seal and wrap in foil. Incubate plate at room temperature on a microplate shaker...each row of a 96-well ELISA microplate. Cover the plate with a 96-well plate seal and incubate at 37 °C... to each well. Cover the plate with a plate seal and incubate on a microplate shaker set to 400 rpm for...

  20. CRISPR Library Amplification

    Type
    Protocol
    ...overnight. Plate 2.5 mL of the transformed cells on each of the eight bioassay plates (two plates per tube...Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least one day in advance... Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser...100 dilution). Plate 100 µL of each dilution onto a prewarmed Petri dish. Incubate plates at 30 ℃ overnight... edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight... are not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12...LB to scrape bioassay plates and remove bacteria. Use one scraper for all plates. Use two 10 mL pipettes...
Showing: 301 - 320 of 349 results