... CP, Carnes AE. Generic plasmid DNA production platform incorporating low metabolic burden seed-stock ...

... 2,3-N-linked sialic acid4 AAV receptor2 Platelet-derived growth factor receptor (PDGFR) α and β3...

...individual bacterial cells to form colonies on a plate and then we performed colony PCR on the register...

...included in a standard homology directed repair template to facilitate the selection of cells containing...

...thought that it was necessary to develop a single platform to connect talented professionals (who migrated...

...on the other side of the world, communication platforms like Slack make teamwork easier. Trust Ultimately...

...and Mark A. Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene...

... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

...hairpin RNA, or artificial microRNA). Using a DNA template for the RNAi trigger is the most prevalent method...

...technique should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...

.... “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer ...

... expand the self-labeling capabilities of this platform. The Janelia Fluor ligands provide brighter and...

... provided you with feedback or mentorship. The platinum standard for an expert is someone who has cited...

...., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have...

...that can be combined with your gRNA and repair templates of choice though modular cloning steps. The Voytas...

..., S.-F., Ravindra Kumar, S., Arokiaraj, C. M., Plattner, V. M., Campos, L. J., Mich, J. K., Monet, D.,...

... the edit should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ...

...High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from...

... an automated ELISA plate washer. Seal the plate. Place the plate on a microplate shaker set at 400 rpm...use an automated ELISA plate washer. Seal the plate Place the plate on a microplate shaker set at 400 rpm... the plate with a plate seal and wrap in foil. Incubate plate at room temperature on a microplate shaker...each row of a 96-well ELISA microplate. Cover the plate with a 96-well plate seal and incubate at 37 °C... to each well. Cover the plate with a plate seal and incubate on a microplate shaker set to 400 rpm for...

...overnight. Plate 2.5 mL of the transformed cells on each of the eight bioassay plates (two plates per tube...Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least one day in advance... Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser...100 dilution). Plate 100 µL of each dilution onto a prewarmed Petri dish. Incubate plates at 30 ℃ overnight... edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight... are not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12...LB to scrape bioassay plates and remove bacteria. Use one scraper for all plates. Use two 10 mL pipettes...