...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...

...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is...resistant to CcdB, as the native ccdA will be present. Most common cloning strains of E. coli do not contain...ever more important to ensure that you choose the most appropriate method for your own experiments. Please...

... expression, you are almost certainly working with an endogenous locus. The most straightforward way to...function at the post-translational level, unlike most of the other mechanisms described. Fig. ...

...Well, that’s just for DNA – right? RNA performs almost all of its biological functions in our cells in...transcription or as inverted repeats. These elements are almost always epigenetically silenced, but dysregulation...RNases in mammalian cells (RNase H1 and H2) handle most of these intermediates, with each one acting on ...

... RNA samples. RNA is inherently less stable than most macromolecules used in scientific research such ...with post translational modifications. One of the most common of these is 2’-O-methylation, where a methyl...major drawback of CRISPR-Cas9 technology is that most expression systems are constantly “on”. There is...

...     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary antibodies you ...antibody as well.  Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain...convenient site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have...

...systems, making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be...compact genome editors and a gRNA down-sized by almost 40%. The activity of Cas12f1 with these modified...of the system to optimize its outcomes. In their most recent work, the Liu Lab boosts the efficiency of...

...gives you access to hard data about what steps are most likely to fail. Our laboratory manager would definitely...interested to see which part of her procedures are the most failure prone. In an academic lab, a similar situation...capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied to ...

...requested in the call. Step 2: Develop a research plan Most grant applications restrict the space available ...more advice on writing a grant proposal. "Murder Most Foul: How Not to Kill a Grant Application" by Vid...process? What types of writing feedback do you find most helpful? Have a great grant writing tip? Share it...

...T-cells were transferred back into the infants. Almost all patients have achieved persistent immunological...distinguish AAVs as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...

... sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...“goldilocks antibody.” Antibodies are one of the most commonly used research tools in scientists’ pockets...kept in mind our own lab’s purposes. The Wu lab mostly uses whole-mount IHC techniques on rodent brains...

...allowing everything to stay in the correct order. Most Fragmid components are available for request on ...plasmid a name and generate the vector file. Ta-da! Most (180/181) Fragmid components are available in the... be using more than six or seven components — in most cases, that's making two or more plasmids — it may...

...membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample and...the least-abundant protein and working toward the most abundant. If the abundance of your targets isn’t...maximize protein retention. Following this step, most PVDF membranes recommend re-wetting with methanol...

...Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent... is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working to develop CRISPR-based...

...genetic screening experiments”, it is certainly the most robust. In this blog post, we will discuss how CRISPR...libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are provided at a concentration... of next-generation sequencing. Representation - Most libraries contain 3-6 gRNAs per target gene, and...

...how molecular biology impacts worm locomotion. Our most recent models of C. elegans molecular biology are...been incorporated as a non-profit entity and so far most of their funding has come from kickstarter and fundrazr...everyone knows the internet loves cats! This is mostly a project to get our computer folks at the makerspace...

...point during this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite...protein function in the human body?” … and probably most importantly… "how do we know this?” Over the past...represent one aspect of the protein very well, it will almost certainly fail to convey other aspects of the structure...

...reference sequence. The five contigs with the most total reads are most likely to correspond to the contaminant...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

...because you will be part of a team in industry with most team members giving oral presentations, you will...to work on later. For instance, AstraZeneca posts most of its positions all at once at the end of February...method of posting varies between companies. Like most jobs, it does help if you know someone who’s already...

... be difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab...though expressing the chromoproteins from the chromosome could get around this problem. There is no single... “ideal” chromoprotein plasmid that combines the most desirable features: intense color, fast maturation...