...methyllysine binding domain (chromodomain), which binds selectively to lysine-methylated peptides. This construct...

... We also offer ready-to-use AAV preparations of select plasmids; these are noted with the viral service...sugar-binding protein into a FRET sensor with high selectivity for sucrose. J Biol Chem. 2006 Oct 13. 281(41..., NTS, UCN, SST, and CRF A tool kit of highly selective and sensitive genetically encoded neuropeptide...Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning...Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. Mol Cell. 2019 Apr 18;74(2):347-362...

...protein of interest, it can be degraded by this selective degrader. The novel degrader, dTAG13, is a small...

...started, too much time for this project was spent selecting guides, designing primers, and running analyses...

...challenge of attempting to simultaneously and selectively induce both cutting and regulation of expression...

... resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies...

...and tsPurple chromoproteins to create negative selection markers for allelic exchange. These vectors contain...

... Plasmids and Resources page, investigators may select a pathway and identify any genes within that pathway...

... Addgene. Addgene performs Sanger sequencing on select regions of all distributed plasmids as part of ...

...and the PITCh- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify...

...Stelzer’s group described the single-plane or selective-plane illumination microscope (SPIM). In order...

... a fitness cost and are eliminated by natural selection, gene drives distort inheritance in their favor...

... complex functions, including the ability to selectively activate/repress target genes, purify specific...additional Cas proteins and PAM sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9...There are many online tools available to help you select an optimized gRNA for your application (see: Plan...is generally low (< 10% of modified alleles). Selecting a target cut site as close as possible to the ...design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity and low ... available in two formats: as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are...

..., Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer...

...specifically cuts methylated DNA, so it will selectively destroy the template, but not newly synthesized...

...   Fig. 2: Students in an MDH CURE replaced select residues of human mitochondrial MDH (hMDH2) with...

...Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably, some information...

...start can save a lot of trouble in the long run.  Selecting the method Once you’ve answered the above questions...

...linearly distant regions close together. gRNA Selection and Filtering for Off-target Effects We first ...

...make sure to use the appropriate antibiotic for selection.   Lysing bacteria and setting up PCR reactions...