... composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed...

...taking care of that”, because that can indicate confusion around... Ownership: Misunderstandings about who...

...less interference with the oligomeric state of a fusion partner. Therefore, we recommend using miRFPs and...

...available from Addgene. This system uses the dCas9-VP64 fusion commonly used to activate transcription, but with...

...answer an expert's question than to leave everyone confused about your research because you waded too deep...

...visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely, while others ...

...antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All bind specific ...

...techniques, such as Golgi staining, injection of diffusible labels, or electron microscopy of stained sections...

...dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes...

...actually performed, potentially curtailing future confusion (and hours sifting through paper lab notebooks...

... of the skyline of Acadia National Park. I was confused. It was supposed to be hard. I was mentally prepared...

... antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...

...Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz, A., Tavakoli, S., Widrick, J. J., Messemer, K...

...TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding Prefix alleviates RBS-CDS issues. Prohibited...6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction... 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts prefix to preserve...

... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

...axonal transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport...

...minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF...

...), or luminescent opsins, are opto-chemogenetic fusion proteins of a light-emitting luciferase and a light-sensing...modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs...

...library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the...expressing and purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

...variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance... of pULTRA. Moore 19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd...pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and 36086 for other FP variants...