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  1. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

  4. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used...

  8. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...been adapted to fluorescently label a sequence matching its gRNA, facilitating the live study of chromatin...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

  11. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...

  12. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

  13. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...outside the box, be creative, and put your inner child to work. If you have an odd or quirky lab story ...

  16. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

  18. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...
Showing: 301 - 320 of 646 results