...address the research challenge. Now is the time to make bold statements. While some of the statements may...6-12 months to give feedback on the application. Make sure to invest time and consider the reviewer's ...the next application. Although some comments may make it clear that the reviewer misunderstood the point...that was being made, take it as an opportunity to make clearer statements on the next round of applications...

...changes (Figure 1), but it can be challenging to make these base changes, especially in primary human ....20-m to edit primary human T cells efficiently makes it a promising tool for T cell therapy development...robust editing in human cell lines and primary cells makes them a great addition to the base editing toolbox..., Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed...

...for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix, store, and...     How are Recombinant Antibodies Made? To make recombinant antibodies, you first need to know the...  Second, plasmids are easy to store and share, making recombinant antibodies the most practical choice... encoding antibodies, scientists will be able to make higher affinity antibodies, modify antibody function...

...Tweeting and texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy... to consider before you commit: Double check to make sure the answer to your question didn’t come with...expedite and improve your customer service experience: Make sure you’re contacting the correct company. This...as possible about the issue. For Addgene support, make sure to mention the plasmid catalog number, or at...

...Jacob Corn contacted us in 2014 to ask if we could make a model of Cas9 based on the structure published...Jennifer Doudna’s new book A Crack in Creation. Making models more useful through flexibility It is common...the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-target hits in a ...our hands-on teaching tools that are designed to make the molecular world real for students. Many thanks...

...limiting reagent for my experiments, so I always make more AAV than I think I need. Unlike lentivirus,.... If you’re new to creating gradients, practice making the iodixanol layers before doing your first purification...purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR titering...Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals...

... jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists negotiate...were hoping that your lab mate would have time to make comments on your manuscript. If you give her this...practice and hone your skills. This practice will make negotiating feel more natural. Creative negotiation...the person/team for help? What can I offer them to make it a win-win situation instead of just extra work...

...to help make the most of these pages, we've written this post to guide you through them and make the best...can be found in this location as well. These maps make it easy for scientists to see if the plasmid would...their plasmids are used in a publication. Addgene makes this easy by providing a template citation for you...

...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...control gene expression. Base editors can help you make precise point mutations near the gRNA target site...single-stranded DNA oligonucleotide, so you will need to make sure your expression system and delivery methods...Envelope plasmids provide the necessary components to make lentiviral particles. Stable, tunable expression...

...messy, so you want to make sure you have sufficient volume to dip all your plants. Make sure your Agrobacterium...agrobacterium: Getting a transformant takes a long time. Make sure the agrobacterial culture you are growing definitely...increase background growth of non-transformants and make finding true transformants more difficult. Don’...

...Science: What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very...topics without some editorializing, and he wisely makes no such attempt. He is transparent about his own...be shared by fellow scientists--but that doesn’t make his introduction to these topics any less effective... revelations. The issues he discusses have been making the rounds in journal editorials, science social...

...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide an...the concentration of the unknown sample.  How do I make the standards for a standard curve? You create a...with BioRender.com.   When choosing a standard, make sure that the sample is pure and free of any contaminants...start with a higher concentration of standard or make smaller dilutions.        As mentioned above, it...

...chemists and biochemists have developed techniques to make labeling chemistry easier and more functional in...and continual improvement of fluorescent proteins makes them the go-to choice for many (if not most) fluorescence...labeling strategies and improved fluorophores are making chemical dyes increasingly attractive and accessible...Medical Institute. He would rather be in the lab making new fluorophores for advanced imaging modalities...

...lab-use tablet? The software also makes it easy to annotate protocols, make them public for others to use,...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA...bench, keep your inventory at your finger tips, and make shopping lists as you finish the last of a reagent...

...yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating...months. But certain models remain more difficult to make. For example, large insertions, like a fluorescent... site. If you are using the ivTRT method, try to make the first two bases of the left homology arm guanine...activity. To avoid re-cutting by Cas9 after editing, make sure that the repair template does not contain the...

...larger target, such as a protein. Such flexibility makes aptamers ideal candidates for biosensors. Aptamer... of how they actually “report” as biosensors. Making aptamers glow The flexibility of aptamers means...rather than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...decrease background in vivo. The nature of aptamers makes them excellent candidates for developing assays ...

...nowadays, we cannot make it a full month without a new, drug-resistant ‘superbug’ making headlines in one... develop survival mechanisms. Some microbes even make their own antibiotic compounds. In such a case, ...resistant bacteria to over-take the population and making them more difficult to treat in the future. Patients...

...their initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and.... Using computational observations, Koonin and Makarova found that Cas7-11 appeared to have derived from...generation came Prime editing — the ability to now make any small changes, including base edits, insertions...Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable...

...grumpiest of researchers can enjoy it at times! What makes it rewarding is when you can tell a trainee is understanding...to show up as your most prepared, can-do self and make the most of it! So, what can you do to build a ...reviewed above. Offer to help clean up and/or make necessary reagents for the experiments. Unlike in...need help with…now”. Ask for feedback so you can make adjustments. This could look like “What are some...

...pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are custom viral ...verified!–your pooled library DNA, the next step is to make your virus. If the library happens to have a specific...vary with titer, cell type, and other factors, so make sure to put that aliquot to full use! Common conditions...chances that a cell doesn’t get infected at all. To make sure that every plasmid’s payload is adequately ...