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  1. TALEN Guide

    Type
    Collection
    ...can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove...

  2. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...FRET More Fluorescent Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics...

  3. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...enChIP technology using recombinant CRISPR ribonucleoproteins. Fujita T, Yuno M, Fujii H. Genes Cells. ...

  4. Viral Production

    Type
    Collection
    ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...native protein complexes. This vector serves as a backbone to clone the left and right homology arms. More...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009...

  10. Rett Syndrome

    Type
    Collection
    ...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources...

  12. The Pleiades Promoter Project

    Type
    Collection
    ...Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen ...

  13. CRISPR Library Amplification

    Type
    Protocol
    ...enzyme for digest that will cut the shared plasmid backbone a single time and visualize that digestion on ...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ...targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...GFP CMV ALS Juan Bonifacino 172202 eGFP-KIF5A-Rigor KIF5A GFP CMV ALS Juan Bonifacino 173579 Lenti-sgAtm...strep KIF5A mCherry, Streptavidin CMV ALS Juan Bonifacino 120538 pSV40_HA-mPKR EIF2AK2 SV40 VWM disease...

  16. Genetic Code Expansion

    Type
    Collection
    ...pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial TAG Thomas Huber 163916...

  17. CRISPR Guide

    Type
    Guide
    ...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

  18. Cloning

    Type
    Guide
    ...Techniques You may also like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme...compatible restriction enzymes, that cut your insert or backbone at only the desired location for your cloning ...putting it under a variety of promoters and into backbones with different selection cassettes). Although ...disadvantage of TOPO® cloning is that very few plasmid backbones are available TOPO® ready, and it is not feasible...overhangs can be efficiently ligated into any plasmid backbone independent of how it was originally designed ...

  19. Molecular Biology Reference

    Type
    Guide
    ... for an empty plasmid backbone for your experiment, see Addgene's empty backbone page for more information...of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can...unique and not located elsewhere in the plasmid backbone, which is why they can be used for cloning by ...Antibiotic Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting sanger...

  20. Chemogenetics Guide

    Type
    Guide
    ...23303063 (Link opens in a new window) Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez...RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M (2017). Chemogenetics revealed:...Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan...
Showing: 281 - 300 of 306 results