...Cultures should only be passaged 20-30 times before discarding and thawing a fresh vial. In many labs, the history...Routinely test your cell line for mycoplasma and discard it if the culture has become overly confluent or...ensure high quality and reproducible results always care for your cell lines and virus properly and be consistent...

...in vitro translation system. The gene set was carefully chosen to perform statistically relevant benchmarking...conveniently performed through the N-terminal eGFP tag carried on all constructs. The Cell-Free Protein Expression... 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F bridging primer...

...’s which allow researchers to deliver oversized cargo (Hirsch et al., 2010). Today’s blog post will give... https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho LS, Turunen HT, Wassmer SJ, Luna-Velez MV, Xiao...transduction following recombination between AAV vectors carrying parts of a larger gene. Nat Biotechnol 20:697–...

...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate... B., Fong, D. M., Mouravlev, A., Young, D., & O’Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy....2014.00076 Nieuwenhuis, B., Haenzi, B., Hilton, S., Carnicer-Lombarte, A., Hobo, B., Verhaagen, J., & Fawcett...

...al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to...0092-8674(84)90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design... JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production platform incorporating...

...latter would likely be more effective, so long as care is taken in the interpretation of results by requiring...use of CRISPR technology will always depend on careful experimental design, execution, and analysis. ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

...health sector, phages represent a reservoir of carbohydrate binding proteins waiting to be explored and ...spot this protein directly onto nitrocellulose and carry out the steps from blocking the membrane to probing...bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion in structural biology 34 (...

...lesser than the mean of a control group. If you only care if one group is different in a certain direction...rate by only performing the comparisons you really care about. Family-wise error is the probability of a...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

...population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to differences in the ...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for... 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ, Cook WH, Young D (2021) AAV Targeting of Glial...

... compartments in in vivo model systems, so extra care must be taken to assess if proper staining is achieved... such as conjugation with proteins, should be carefully managed. Proper dye-to-protein ratios, or the ... and quenching, which can be mitigated through careful selection and optimization. Fluorescent experiment...

... interpreting your data therefore necessitates careful design of RNAi sequences, confirmation of RNA knockdown...doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...

...Vectors Available at Addgene: Lab Plasmids Dan Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis...target cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently...there are some drawbacks with this technique. Great care needs to be taken with common S288c strains since...apicomplexan genes remain uncharacterized. Recent studies carried out by Sebastian Lourido and colleagues constitute...

...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

...Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author...Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author: Benedict...

...Chew! Podcast breakdown Intro: Wei Leong Chew's career path and why he chose to go into genome engineering...Chapter 7/Outro: Advice for researchers pursuing careers in academia. Many thanks to our guest, Wei Leong...

...resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry Schaefer, ...reach out to me by email.  About the author Niko McCarty is the editor of Asimov Press and is designing ...

...Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going into academia: follow...

...about how to navigate through all stages of your career, whether you’re a graduate student, post-doctoral...   Caroline LaManna...

...re selected as a winner, you’ll receive a winter care package from Addgene.  Here are the details:   ...  Caroline LaManna...

...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...