Addgene

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Showing: 281 - 300 of 424 results

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Mammalian RNAi Tools

Type

Collection

...modifying susceptibility to type 1 diabetes. Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker...
Antibodies 101: Fc Effector Functions

Type

Blog Post

...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...
CRISPR 101: Drug discovery and CRISPR

Type

Blog Post

...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...
Plasmids 101: Gene, enhancer, and promoter trapping

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Blog Post

...DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020...
New and Upcoming Viral Vectors - Spring 2019

Type

Blog Post

... Plasmid Serotype Name 50363 AAV5 AAV phSyn1(S)-DreO-bGHpA 121675 AAV9 pAAV-EF1a-fDIO-Cre (FLP-dependent...
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

Type

Blog Post

...134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Type

Blog Post

...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly...
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Type

Blog Post

...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Type

Blog Post

...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ...
Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

...resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect...
New and Upcoming Viral Vectors - September 2019

Type

Blog Post

...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for...
Visualizing Translation at the Single Molecule Level

Type

Blog Post

...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...
The Power Behind NGS Plasmid Validation: seqWell

Type

Blog Post

...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...
Hot Plasmids and Viral Preps - September 2021

Type

Blog Post

...DNA repair. A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...
In Vivo Biotinylation of Bacterial Fusion Proteins

Type

Blog Post

... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized...
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Type

Blog Post

...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ...
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Type

Blog Post

...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the...
Don’t Wait To Launch Your Health Science Startup

Type

Blog Post

...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using...

Showing: 281 - 300 of 424 results

Results per page:

Mammalian RNAi Tools

Antibodies 101: Fc Effector Functions

CRISPR 101: Drug discovery and CRISPR

Plasmids 101: Gene, enhancer, and promoter trapping

New and Upcoming Viral Vectors - Spring 2019

Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Antibodies 101: Designing Your First Flow Panel

Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Plasmids 101: Gram Negative and Positive Bacteria

New and Upcoming Viral Vectors - September 2019

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Visualizing Translation at the Single Molecule Level

The Power Behind NGS Plasmid Validation: seqWell

Hot Plasmids and Viral Preps - September 2021

In Vivo Biotinylation of Bacterial Fusion Proteins

HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Don’t Wait To Launch Your Health Science Startup

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