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1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   Blog Post

   ...1):81-89. PubMed. 2. Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer...

2. The Stingy Scientist: How the Baby Gel Box Was Born

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   Blog Post

   ...need them.  When I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need...

3. Arf GTPase Family

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   Collection

   ...Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi...

4. TALEN Guide

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   Collection

   ...from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...

5. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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   Blog Post

   ...PubMed PMID: 24727401. Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural... PubMed Central PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted...

6. New and Upcoming Viral Vectors - Spring 2019

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   Blog Post

   ... Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent...

7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

   ...134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe...

8. Antibodies 101: Fc Effector Functions

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   ...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...

9. CRISPR 101: Drug discovery and CRISPR

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   Blog Post

   ...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...

10. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post

    ...DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020...

11. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post

    ...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly...

12. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ...

13. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ...

14. Antibodies 101: Designing Your First Flow Panel

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    Blog Post

    ...global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of...

15. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ...

16. New and Upcoming Viral Vectors - September 2019

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    Blog Post

    ...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...

17. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    ...is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for...

18. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

    ... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized...

19. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...

20. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...