...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...

...molecular technologies!   References 1. Demaneche, S., et al “Laboratory-scale evidence for lightning-mediated...

...DNA repair.  A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...

...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the...

...resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect...

...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...

... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized...

.... and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has ...

...complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...

...multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID: 25831507. PMCID: PMC4394297...

... introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and yielded an...

...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those...

... Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B.... One method uses orthogonal dCas9’s (e.g., S. pyogenes dCas9 and S. aureus dCas9) tagged with different...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan...endogenous gene(s) without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting...AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, Itier...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...Overview Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems, which ...

...This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...

...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

...edition.  References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ...

...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-...

... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...

...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts...