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Showing: 261 - 280 of 349 results

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CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

...Consortium, also known as the Genetic Perturbation Platform, to produce their shRNA library to support functional...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

...100% efficient at halting transcription of the template and initiating the desired cleavage event, although...
Plasmids 101: Protein Expression

Type

Blog Post

...expression consists of transfecting cells with your DNA template of choice and allowing these cells to transcribe...
3D Printing Meets CRISPR Cas9

Type

Blog Post

...cleavage”. Jacob computationally added the non-template strand to the Jinek structure so that the resulting...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...
Tips to Make the Most of a Scientific Conference

Type

Blog Post

...’s best to turn to an established social media platform. During the conference If the conference has a...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...
MXS Chaining

Type

Blog Post

...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

Showing: 261 - 280 of 349 results

Results per page:

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Popular Retroviral Vectors and Their Uses in Scientific Research

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: Terminators and PolyA signals

Plasmids 101: Protein Expression

3D Printing Meets CRISPR Cas9

AAV Vector Quality Control: Going the Extra Mile with NGS

Tips to Make the Most of a Scientific Conference

Addgene's Cancer Collection Pages Connect the Research Community

Production of Virus in Insect Versus Mammalian Cells

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

AAVs CREATed for Gene Delivery to the CNS and PNS

Isolating Genomic Regions of Interest with the CRISPR System

CRISPR 101: Ribonucleoprotein (RNP) Delivery

MXS Chaining

CRISPR 101: RNA Editing with Cas13

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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