We narrowed to 291 results for: nif

1. Data Freedom: The Expansion of Data Sharing in Research Publications

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   ... storing data in a sharable form is indeed a significant effort. Our standards of data recording are highly...

2. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...On this gel, the protein of interest band is significantly lighter in “condition 1” than “condition 2.”...

3. March for Science

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   ...group (15) While marchers are a group with one unifying interest (the advancement and inclusion of science...

4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   ..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...

5. CRISPR 101: Validating Your Genome Edit

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   ...clones, it’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...

6. An Integrin Antibody Toolkit from IPI

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   ...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ...

7. Tips for Getting a Faculty Position

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   ...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

8. Transferable Skills Guide: Conflict Resolution

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   ...owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was X’s job”...

9. Site Directed Mutagenesis by PCR

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   ...figure). Template A high purity plasmid prep significantly increases the success rate of site directed ...

10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, although the ...

11. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...they all start with a G!) but actually work by significantly different methods. Golden Gate cloning does ...

12. How to Negotiate a Successful Lab Start Up Budget

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    ...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

13. Plasmids 101: Common Lab E. coli Strains

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    ... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...

14. Hot Plasmids: Spring 2025

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    ...and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, and sensitivity...

15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...few replicates leading to suspect statistical significance (you can read more about this problem in Button...

16. Antibodies 101: Multiplex Immunofluorescence

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    ...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...

17. Plasmids 101: Restriction Cloning

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    ...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

18. A History of Genome Engineering in Popular Culture

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    ...10.1038/s41586-018-0070-x Griffith F (1928) The Significance of Pneumococcal Types. Journal of Hygiene 27...

19. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...

20. Important Considerations When Using AAVs

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    ...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...