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We narrowed to 281 results for: nif

Showing: 261 - 280 of 281 results

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Site Directed Mutagenesis by PCR

Type

Blog Post

...figure). Template A high purity plasmid prep significantly increases the success rate of site directed ...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, although the ...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...they all start with a G!) but actually work by significantly different methods. Golden Gate cloning does ...
How to Negotiate a Successful Lab Start Up Budget

Type

Blog Post

...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...
Plasmids 101: Common Lab E. coli Strains

Type

Blog Post

... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

...few replicates leading to suspect statistical significance (you can read more about this problem in Button...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...
Plasmids 101: Restriction Cloning

Type

Blog Post

...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...
When Fidelity Matters: A frank discussion about ligase fidelity

Type

Blog Post

...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...
Finding Your Science Policy Path

Type

Blog Post

...depending on how and where you pursue it. It can manifest via research and data gathering, regulatory efforts...
A History of Genome Engineering in Popular Culture

Type

Blog Post

...10.1038/s41586-018-0070-x Griffith F (1928) The Significance of Pneumococcal Types. Journal of Hygiene 27...
Important Considerations When Using AAVs

Type

Blog Post

...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

...should appear less green. Note that there won’t be uniform editing across all the cells in the population,...
Antibodies 101: Introduction to Antibodies

Type

Blog Post

...antibodies. They also have the advantage of being significantly smaller, meaning they can recognize epitopes...
Typing CRISPR Systems

Type

Blog Post

...like to explore Class 2 CRISPR in more depth, the Jennifer Doudna lab maintains an encyclopedia of Class ...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Type

Blog Post

...incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Type

Blog Post

...detail. References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

... Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple...
CRISPR 101: Cytosine Transversion Editors

Type

Blog Post

...processes that would cause DSBs, and its inclusion significantly reduced indels generated by CGBEs. What's next...
Optogenetics + CRISPR, Using Light to Control Genome Editing

Type

Blog Post

...C714, generating paCas9-2. This change did not significantly alter the system’s efficiency at generating ...

Showing: 261 - 280 of 281 results

Results per page:

Site Directed Mutagenesis by PCR

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

How to Negotiate a Successful Lab Start Up Budget

Plasmids 101: Common Lab E. coli Strains

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Antibodies 101: Multiplex Immunofluorescence

Plasmids 101: Restriction Cloning

When Fidelity Matters: A frank discussion about ligase fidelity

Finding Your Science Policy Path

A History of Genome Engineering in Popular Culture

Important Considerations When Using AAVs

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Antibodies 101: Introduction to Antibodies

Typing CRISPR Systems

Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

CRISPR 101: Multiplex Expression of gRNAs

CRISPR 101: Cytosine Transversion Editors

Optogenetics + CRISPR, Using Light to Control Genome Editing

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