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  1. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...Gersbach Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA... CRISPR Gersbach Lab CRISPR Plasmids Gersbach...CRISPR Cas9 gRNA plasmids from Dr. Charles Gersbach's lab. CRISPR...gRNA Design Tools CRISPR Blog Posts These mammalian expression plasmids encode the nuclease-null dCas9 ...are described in: RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera ...cells. We also provide a collection of reagents for CRISPR/Cas9-based multiplex genome engineering in diverse...This set of plasmids is described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral...

  2. Plant Plasmids and Resources

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    ... Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors,...technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants... gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose Toolkit enables...truncatula , wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species...series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes...

  3. Dimeric CRISPR RNA-guided FokI Nuclease (RFN)

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    Collection
    ... CRISPR Joung Lab CRISPR Plasmids CRISPR-FokI Dimeric...Dimeric CRISPR RNA-guided FokI Nuclease (RFN) Technology You may also like... CRISPR Guide CRISPR Protocols...Design Tools CRISPR Blog Posts This page lists plasmids for practicing the dimeric CRISPR RNA-guided FokI...lab Dimeric CRISPR RNA-guided FokI Nuclease (RFN) CRISPR...

  4. Kamoun Lab CRISPR Plasmids

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    Collection
    ...Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design... CRISPR Kamoun Lab CRISPR Plasmids Kamoun Lab...Design Tools CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work in the model plant Nicotiana...CRISPR Cas9 gRNA plasmids from Dr. Sophien Kamoun's lab. CRISPR... mutagenesis in model and crop plants using the CRISPR/Cas system. Belhaj K, Chaparro-Garcia A, Kamoun...

  5. Bikard Lab - CRISPR Repression Collection

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    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently... DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9...collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp with either...

  6. Microbiology Resources

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    ...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific... Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression... specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology : Browse our deposited...editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering blog post...

  7. CRISPR Plasmids - Xenopus

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  8. Doudna Lab CRISPR Plasmids

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    Collection
    ...Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design... CRISPR Doudna Lab CRISPR Plasmids Doudna Lab...CRISPR Cas9 gRNA plasmids from Dr. Jennifer Doudna's lab. CRISPR...Design Tools CRISPR Blog Posts Assorted Cas9 nucleases from different bacteria as well as a mammalian codon-optimized...Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft ...

  9. Huang Lab CRISPR Plasmids

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    ...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A human codon-optimized... CRISPR Huang Lab CRISPR Plasmids Huang Lab CRISPR...CRISPR Cas9 gRNA plasmids from Dr. Xingxu Huang's lab. CRISPR...

  10. Guide RNA Expression Plasmids for EGFP

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    Collection
    ... CRISPR Joung Lab CRISPR Plasmids EGFP gRNA CRISPR-Cas/RGN expression plasmids...EGFP You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab...Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify three different...

  11. CRISPR Plasmids - Base Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused ...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR base editing plasmids. Plasmids are available for...

  12. CRISPR Plasmids - C. elegans

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR C. elegans CRISPR Plasmids: C. ...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  13. Guide RNA Expression Plasmids for Endogenous Human Genes

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    Collection
    ... CRISPR Joung Lab CRISPR Plasmids Human gRNA CRISPR-Cas/RGN expression plasmids...genes You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab...Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify four different...

  14. CRISPR Plasmids - Drosophila

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional... functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based...Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  15. CRISPR Plasmids - Bacteria

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...mutations. Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and ...

  16. Qi Lab CRISPR Page

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    ... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...or human cells ( Qi et al. ). This CRISPR interfering system (CRISPRi), works independently of host cellular...uniqueness of CRISPRi, as compared to several recently published works on applying the wild-type CRISPR system...recently repurposed the bacterial immune system, CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...genes. These plasmids are described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific...

  17. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ... you are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts... Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular...

  18. Guide RNA Expression Plasmids for Endogenous Zebrafish Genes

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    ... CRISPR Joung Lab CRISPR Plasmids Zebrafish gRNA CRISPR-Cas/RGN expression plasmids...Zebrafish You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab...Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify nine different...

  19. Zebrafish Plasmid Collection

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    ...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...

  20. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses...
Showing: 261 - 280 of 438 results