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We narrowed to 326 results for: cloning

Showing: 261 - 280 of 326 results

  9. Zinc Finger Consortium Reagents

    Type
    Collection
    ... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

  10. Viral Vectors

    Type
    Collection
    ...choice (Fig. 1B) . Thus, through standard plasmid cloning, viruses can be engineered to harbor a wide array...easily modified and amplified (through standard cloning methods). There are different types of viruses ...

  11. Video Library

    Type
    Protocol
    ...technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an...

  12. TALEN Expression Vectors

    Type
    Collection
    ...using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about...

  13. CRISPR Plasmids - Plants

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...

  14. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do...Chain Reaction (PCR) You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation...

  19. Antibody Guide

    Type
    Collection
    ...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies...

  20. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... Protocols Cloning by Limiting Dilution Isolating a Monoclonal Cell...transgene expression over time, as the lower expressing clones take over the polyclonal cell pool. Generating ...new window) or isolating individual cells with cloning cylinders. Workflow Timeline This protocol begins...
Showing: 261 - 280 of 326 results