We narrowed to 324 results for: cloning

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

Protocol - How to Ligate Plasmid DNA

Transducions use viral vectors to insert nucleic acids into cells. Learn all about them in our Viral Vectors 101!

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

Learn more about how to verify your plasmid via a diagnostic restriction digest.

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Learn about AlphaFold and how you can use it for protein structure predictions to inform interesting research questions.

Guide to TALEN technology and using TALEN plasmids

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

Ginkgo Bioworks and Addgene are synthesizing and distributing thousands of plasmids for COVID-19 research. Read to learn more about these constructs

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and explain common applications.

A deep dive into qPCR with a focus on analysis, chemistries, and bench tips.

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Check out how you can use digital PCR to validate your CRISPR genome edits.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The collection can be used for screening transcription factors for cellular programming or cancer drug resistance.