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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 333 results for: cloning

Showing: 261 - 280 of 333 results

  3. Worm Expression Resources

    Type
    Collection
    ...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6...

  5. Video Library

    Type
    Protocol
    ...technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an...

  6. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do...Chain Reaction (PCR) You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with ...Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional... for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE...

  9. Synthetic Biology - Strains

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ...

  11. Synthetic Biology - Metabolism

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  13. Zinc Finger Consortium Reagents

    Type
    Collection
    ... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

  18. CRISPR Plasmids - Plants

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...

  19. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... Protocols Cloning by Limiting Dilution Isolating a Monoclonal Cell...transgene expression over time, as the lower expressing clones take over the polyclonal cell pool. Generating ...new window) or isolating individual cells with cloning cylinders. Workflow Timeline This protocol begins...
Showing: 261 - 280 of 333 results