We narrowed to 582 results for: arl
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TypeBlog Post...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...
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A Guide to Starting Your Own Journal Club
TypeBlog Post...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions close... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...easier to find materials and learn about them. As early as 2007, we began grouping similar tools together...12 guides available on our website, which are regularly used by scientists around the world. Collectively... -
Hot Plasmids Spring 2024
TypeBlog Post... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...article is an interactive chart where you can see the yearly interdisciplinarity index of research for single...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...major effect on scientists within academia, most particularly postdocs working in the U.S., whose current ...thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical research... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...reprogramming from 0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now... -
Career Insights: Technical Support Specialist
TypeBlog Post... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...conferences should strive to provide support to early career scientists, students, and those from historically...Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...and I met scientists from 21 different labs who clearly also share this wonder of the organism. It’s...songs like humans learn speech. Finches must learn early, use auditory feedback to learn, babble as baby ... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments... of cookie monster mutant flies are filled with early mitotic cells as well as necrotic or dead cells ...