We narrowed to 601 results for: arl
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TypeBlog Post...expression cassette in one single viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas...of AdV-modified human cells being subjected to scarless homology-directed genome editing (Maggio et al...
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Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...creates chimeric circles of DNA that are distant linearly but close together in space. The chromatin “picture...enhancer is interacting with. Super enhancers are particularly good models for building chromatin interaction... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...dsDNA in most starting materials, and is not particularly sensitive. The fluorescent signal can be affected...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...may be too constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens...your CRISPR screen to a high degree. This is particularly helpful when investigating tissue-specific diseases... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life. Resources More resources on the Addgene... -
Academic vs. Industry Postdocs
TypeBlog Post...experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...seen some major advances in light microscopy, particularly related to the neurosciences. These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions close... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...and I met scientists from 21 different labs who clearly also share this wonder of the organism. It’s...songs like humans learn speech. Finches must learn early, use auditory feedback to learn, babble as baby ... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments... of cookie monster mutant flies are filled with early mitotic cells as well as necrotic or dead cells ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...reprogramming from 0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now... -
Career Insights: Technical Support Specialist
TypeBlog Post... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ...