Skip to main content
Addgene
Showing: 261 - 280 of 560 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune...

  4. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...article is an interactive chart where you can see the yearly interdisciplinarity index of research for single...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources...

  5. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...major effect on scientists within academia, most particularly postdocs working in the U.S., whose current ...thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical research...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...

  9. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    ... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    ... is still a problem today. It is now known that nearly 500 cell lines suffer from contamination with HeLa...out on low passage cells and this means you can regularly discard the cells you are working with because...

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...American Type Culture Collection and freeze as many early passage vials as possible. One problem that often...can make this assumption incorrect. One of the earliest steps in lentiviral transduction is the interaction...

  18. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...while requiring a lower concentration of sample — nearly ten times less protein. While the addition of LEAs...Methyltransferase (CHARM), with several advantages over the earlier system.  Rather than overexpressing an exogenous...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...distancing. With countries like South Korea, one of the earlier epicenters of the outbreak ultimately flattening... is a one-pot, one-temperature reaction, and is nearly single-molecule sensitive. In the case of HIV-1...
Showing: 261 - 280 of 560 results