...heterogeneous cultures.  HeLa cells expressed a fusion of keratin with mGold (top) and mVenus (bottom)...

...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

...activated through administered drugs that must diffuse throughout the body, the onset of receptor activation...

...Uncx Mouse Mouse IgG2a 114547 Anti-Mitofusin-2 [N171/17.4R] Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin...SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a 140073 Anti-Pannexin...Mouse IgG2a 225377 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a...IgG2a 225378 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a.../Tmem90b Rat Mouse 211603 Mitofusin-1 scFv [N111/24] N111/24 scFv Mitofusin-1 Rat Mouse 211604 Botch scFv...Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising a κ light chain...heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion...

... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...

...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...

...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources...

... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...

...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...

...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...

...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...

...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...

...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable...

... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime...

...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students...

...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...

... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...

...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining...

... Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...