...visited Cambridge to spend some time learning and doing research at Novartis. Listen to learn how Kwabena...

...of proteins functioning in a variety of ways. How do scientists focus on the activity or function of a...plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing a polyhistidine...perform assays that they otherwise would not be able to do. We hope that this blog has answered all of your ...

... synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce...many suitable dyes to choose from and you can opt to do-it-yourself with a kit or purchase it commercially...but you can still choose what dye to use  What to do once you’ve chosen a gRNA and modification(s) Congratulations...

...you have all this knowledge, the question is: How do you know what’s too much (or too little)?  The answer...things aren't working out, adjust the ask.  I used to do a lot of outreach to student groups and being aware...think about everything you’re asking your learners to do, it becomes a lot easier to build successful, accessible...

... little more about how I ended up at pgEd, what I do there, and how you can set yourself on a path towards... affiliation, and ethnic or personal identity. We do this through a variety of activities, including creating...newspapers, or starting your own blog. Even if you do not end up pursuing a career in science communication...

... plans to raise salaries. What are institutions doing after the injunction? We are particularly interested... have found out about what their institution is doing from Future of Research over social media, not from...recommendations, even when a federal mandate encourages them to do so. Immediately after the injunction, as we started...

...procedure dramatically reduces the number of clones that do not contain the recombinant plasmid and therefore...are identified much more efficiently, as those that do not contain the desired insert should not grow.   ...be present. Most common cloning strains of E. coli do not contain the F plasmid (and are considered F-)...

...without a kit that outlined several reasons why doing something without a kit has advantages: less plastic...without saying that one should take care whenever doing any type of DNA or RNA purification to avoid contamination...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful...

...cells - a rich soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming...mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread and drug resistance...for a tumor to spread and to resist drugs. And to do that, let’s take a look at exosomes. Proficient in...

...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...just a question you have about the world. You might do some background research to better understand it ...method to problems in a variety of contexts.  How do you develop problem solving skills? Thankfully, as...

...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to ...mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can be used...

...technologies and spaces where interested parties can do research. This interest manifested itself in the ... are on the lookout for genome modifications that do not exist in the CGC library but that would be useful..., we have members with a wealth of skills to help do science on the cheap using equipment that would otherwise...

...unlikely you’ll be able to do them all for every western blot assay you run. How do you know which steps to...visualize them with a conjugated antibody. If you do not need such precise measurements, you can use a... double ladder on the lefthand side. Whatever you do, be consistent and document it.   Did it work? Save...directly from tissue or supernatant, and you’re doing the purification and cross-adsorption in lab, consider...adjusting your exposure time first, since you can do that without having to re-run the western.  Remember...the data and what level of accuracy you’ll need to do that confidently.  Once you’ve run your western, ...

...Additional Resources AAV Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for...

... Reddit AMA Here are some concrete things you can do to develop those transferable skills while you are...relies on a division of labor Serve on a committee and do something big like plan a conference Recruit many...

...csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when planning...Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides...knock-in efficiency (<2% positive by ICC for the tag) or do not see any cells positive for tag insertion, you...et al. 2018), however, if you have any questions, do not hesitate to contact me at [email protected]...

...insertions that could compromise function. When we do find mutations, we check to see if they affect the... confirms that these differences are expected and do not affect plasmid function, we will update information... They also differ from the CPS process in that we do not fully sequence all plasmids in the pool but instead...

...you, you may wonder which antibody should I pick? Do I go for the rabbit or the mouse? Is mouse IgG1 better...signal strength (Fig. 1). Monoclonal primaries If you do decide on a monoclonal antibody you may wonder, “...immunoglobulins and reduce the background staining but they do not always eliminate the issue (Lu 1998). Consequently...

...templates for the cloning process. Time spent actually doing the experiments are also generally comparable so...inexpensive DpnI enzyme. Otherwise you let your E. coli do the work for you. Traditional restriction enzyme ...are never any guarantees, some cloning techniques do tip the scales in your favor. For starters, Golden...

... at this still relatively small nonprofit. If you do go longer than a few days without getting a response...particularly when it comes to technical questions. BUT, I do believe that scientists who contact customer service...Our Frequently Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help...