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We narrowed to 768 results for: Dos

Showing: 261 - 280 of 768 results

  1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to ...mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can be used...

  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...technologies and spaces where interested parties can do research. This interest manifested itself in the ... are on the lookout for genome modifications that do not exist in the CGC library but that would be useful..., we have members with a wealth of skills to help do science on the cheap using equipment that would otherwise...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...unlikely you’ll be able to do them all for every western blot assay you run. How do you know which steps to...visualize them with a conjugated antibody. If you do not need such precise measurements, you can use a... double ladder on the lefthand side. Whatever you do, be consistent and document it.   Did it work? Save...directly from tissue or supernatant, and you’re doing the purification and cross-adsorption in lab, consider...adjusting your exposure time first, since you can do that without having to re-run the western.  Remember...the data and what level of accuracy you’ll need to do that confidently.  Once you’ve run your western, ...

  6. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... Reddit AMA Here are some concrete things you can do to develop those transferable skills while you are...relies on a division of labor Serve on a committee and do something big like plan a conference Recruit many...

  7. Plant Plasmids and Resources

    Type
    Collection
    ...thaliana based on RNA-seq analysis. Return to top Do you have suggestions for other plasmids that should...

  8. AAV Packaged on Request

    Type
    Collection
    ...must place your order to begin production. You can do this whenever you are ready. Payment information ...

  9. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when planning...Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides...knock-in efficiency (<2% positive by ICC for the tag) or do not see any cells positive for tag insertion, you...et al. 2018), however, if you have any questions, do not hesitate to contact me at [email protected]...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...you, you may wonder which antibody should I pick? Do I go for the rabbit or the mouse? Is mouse IgG1 better...signal strength (Fig. 1). Monoclonal primaries If you do decide on a monoclonal antibody you may wonder, “...immunoglobulins and reduce the background staining but they do not always eliminate the issue (Lu 1998). Consequently...

  11. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...insertions that could compromise function. When we do find mutations, we check to see if they affect the... confirms that these differences are expected and do not affect plasmid function, we will update information... They also differ from the CPS process in that we do not fully sequence all plasmids in the pool but instead...

  12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...templates for the cloning process. Time spent actually doing the experiments are also generally comparable so...inexpensive DpnI enzyme. Otherwise you let your E. coli do the work for you. Traditional restriction enzyme ...are never any guarantees, some cloning techniques do tip the scales in your favor. For starters, Golden...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...Get your voice out there Here’s one thing you can do today: Sign up for a Twitter account. Check out the...happening. 3) Volunteer – Step into science education Do you aim to reach young people? Try networking with...TED-Ed lesson. 6) Network – Mingle with pros Where do you meet like-minded science communicators? Attend...

  14. Tips for Technical Support Calls

    Type
    Blog Post
    ... at this still relatively small nonprofit. If you do go longer than a few days without getting a response...particularly when it comes to technical questions. BUT, I do believe that scientists who contact customer service...Our Frequently Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help...

  16. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...environment without sacrificing performance or quality. To do this, we can use the ACT label, which is an eco-nutrition...approach to lab waste is something we all need to do. Remember, it was only 50 years ago that we routinely...

  17. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ... SITN, we discuss some of the many things you can do start your own science communication student group...get more involved with your local community. What do Vini and Amy say is the quickest way to get things...

  18. Editor's Choice, September 2016

    Type
    Blog Post
    ...views for the month of September! Historically we do better in September than in the summer months, but...phages are viruses that infect bacteria and that can do so with exquisite specificity (check out our interview...

  19. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...influenced by temporally ordered biochemical events. How do RSMs work? The state of an RSM is defined by the ...live, intact cells; the sequencing and qPCR methods do not. How might one implement RSMs? In our study (...that correspond to the desired inputs. Such sensors do not necessarily have to be based on transcriptional...that best suits your application. How efficiently do RSMs work? We tested a two-input (ATc and Ara) RSM...

  20. Bacterial Expression Systems

    Type
    Collection
    ...transcription factor bind to a particular sequence? Do these two proteins interact? What happens when I ...of modularly assembled zinc fingers. Return to Top Do you have suggestions for other plasmids that should...
Showing: 261 - 280 of 768 results