Addgene

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Showing: 261 - 280 of 413 results

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Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

Type

Blog Post

... al also used arg1 to in vivo image tumour-homing S. typhimurium strain ELH1301. Bourdaeu et al see this...
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

Type

Blog Post

...1):81-89. PubMed. 2. Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer...
The Stingy Scientist: How the Baby Gel Box Was Born

Type

Blog Post

...need them. When I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

...PubMed PMID: 24727401. Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural... PubMed Central PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted...
TALEN Guide

Type

Collection

...from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...
Luciferase Plasmids

Type

Collection

...Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R egion) plasmids... in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc Firefly Insertion of...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Type

Blog Post

...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly...
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

Type

Blog Post

...134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe...
Antibodies 101: Fc Effector Functions

Type

Blog Post

...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...
CRISPR 101: Drug discovery and CRISPR

Type

Blog Post

...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...
Plasmids 101: Gene, enhancer, and promoter trapping

Type

Blog Post

...DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020...
New and Upcoming Viral Vectors - Spring 2019

Type

Blog Post

... Plasmid Serotype Name 50363 AAV5 AAV phSyn1(S)-DreO-bGHpA 121675 AAV9 pAAV-EF1a-fDIO-Cre (FLP-dependent...
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Type

Blog Post

...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Type

Blog Post

...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ...
Mammalian RNAi Tools

Type

Collection

...modifying susceptibility to type 1 diabetes. Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker...
Don’t Wait To Launch Your Health Science Startup

Type

Blog Post

...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using...
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Type

Blog Post

...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ...
New and Upcoming Viral Vectors - September 2019

Type

Blog Post

...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for...

Showing: 261 - 280 of 413 results

Results per page:

Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

The Stingy Scientist: How the Baby Gel Box Was Born

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

TALEN Guide

Luciferase Plasmids

Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

Antibodies 101: Fc Effector Functions

CRISPR 101: Drug discovery and CRISPR

Plasmids 101: Gene, enhancer, and promoter trapping

New and Upcoming Viral Vectors - Spring 2019

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Antibodies 101: Designing Your First Flow Panel

Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Mammalian RNAi Tools

Don’t Wait To Launch Your Health Science Startup

HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

New and Upcoming Viral Vectors - September 2019

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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