...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...

... al also used arg1 to in vivo image tumour-homing S. typhimurium strain ELH1301. Bourdaeu et al see this...

..., Addgene has “literally enabled sharing with 100's of investigators worldwide. Now everything from every...

...photoconvertible proteins. With Sattarzadeh et al.’s discovery that GFP itself is photoconvertible, new...

...efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins...

...1):81-89. PubMed. 2. Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer...

...need them.  When I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need...

...PubMed PMID: 24727401. Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural... PubMed Central PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted...

...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly...

...134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe...

... Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent...

...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...

...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...

...DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020...

...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ...

...209744 pAB228v TrpRS S. cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial...Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran...

...Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R egion) plasmids... in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc Firefly Insertion of...

...from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...

...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ...