... electroporated cells re-adhered to the culture plate within 4 hr of treatment. In some primary cell lines...

...individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and...

...During transcription, RNA is synthesized from a DNA template, inherently generating RNA:DNA intermediates in...

... 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for...

...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...

... The development of the transient transfection platform for assaying transcriptional activity, for example...

...produced. The antisense strand is referred to as the template strand or non-coding strand as this is the strand...promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence... is the RNA responsible for providing a stable template for the translation of a protein. RNA polymerase...

...assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms of its sequencing, in vivo studies...

... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

...Consortium, also known as the Genetic Perturbation Platform, to produce their shRNA library to support functional...

...the topic.  Meta Meta is a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta...

... them to these organisms via another optimized platform. Gene Stacking As the name implies, gene stacking...

...Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from: Gateway...

...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...

... of Glycosylation (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals...

... MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes from...

...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

...100% efficient at halting transcription of the template and initiating the desired cleavage event, although...

...’s best to turn to an established social media platform. During the conference If the conference has a...