We narrowed to 286 results for: nif

1. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

2. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   ...grant seekers to use online applications with significant character limits for the written portion of the...

3. Bioinformatics at Addgene

   Type

   Blog Post

   ...without having to translate or convert programs to a uniform language.  The modern architecture of Nextflow ...

4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   ...CRISPR guide or leave a comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update...

5. Hot Plasmids - November 2023

   Type

   Blog Post

   ...efficient for short, unstructured templates and significantly reduced undesired edits (indels, etc.), while...

6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   ...post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  The advent...

7. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   ...with a particular experiment or project thus significantly enhances the ability of others to subsequently...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   ... Jennifer Tsang...

9. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   ... Jennifer Tsang...

10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    ...displays similar knockdown efficiency but no significant off-target effects, a distinct contrast to the...

11. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    ...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater...

12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    ...is the fact that they occur in the context of significant clinical uncertainties and a rapidly evolving...

13. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    ...create specific edits in DNA, there has also been significant work on constructing Cas9 transcriptional activators...

14. Plasmids 101: Control Plasmids

    Type

    Blog Post

    ...and repeats—what is the difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296...

15. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    ...highest absorbing acceptor and fluorophores with significant overlap in their spectra. The pair CFP-YFP was...

16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    ... storing data in a sharable form is indeed a significant effort. Our standards of data recording are highly...

17. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    ...owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was X’s job”...

18. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...

19. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    ...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ...

20. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...On this gel, the protein of interest band is significantly lighter in “condition 1” than “condition 2.”...