We narrowed to 292 results for: nif

1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. ...

2. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...polymerase activity, whereas some ratios result in significantly reduced activity (4). To ensure that you end...

3. Cpf1: A New Tool for CRISPR Genome Editing

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   ... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

4. A Career in Grants: How To Become a Grant Professional

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   ...grant seekers to use online applications with significant character limits for the written portion of the...

5. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   ... Panda, D., Baig, M. J., & Molla, K. (2025). A unified protocol for genome editing in monocot and dicot...

6. QC Sequencing Technologies at Addgene

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   ...This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley...

7. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   ...This post was written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi...

8. Hot Plasmids - November 2023

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   ...efficient for short, unstructured templates and significantly reduced undesired edits (indels, etc.), while...

9. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...CRISPR guide or leave a comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update...

10. Viral Vectors 101: Pseudotyping

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    ... Jennifer Tsang...

11. Supporting Reproducibility with a Connected ELN

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    ...with a particular experiment or project thus significantly enhances the ability of others to subsequently...

12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...displays similar knockdown efficiency but no significant off-target effects, a distinct contrast to the...

13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ... Jennifer Tsang...

14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  The advent...

15. Bioinformatics at Addgene

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    ...without having to translate or convert programs to a uniform language.  The modern architecture of Nextflow ...

16. Mesothelioma - Causes, Symptoms, and Treatment

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    ...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater...

17. Healthcare Consulting: A Door to the Business of Life Sciences

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    ...is the fact that they occur in the context of significant clinical uncertainties and a rapidly evolving...

18. CRISPR Activation: A Practical Guide

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    ...create specific edits in DNA, there has also been significant work on constructing Cas9 transcriptional activators...

19. Plasmids 101: Control Plasmids

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    ...and repeats—what is the difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296...

20. Tips for Using FRET in Your Experiments

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    ...highest absorbing acceptor and fluorophores with significant overlap in their spectra. The pair CFP-YFP was...